Mutlu Pelin, Mutlu Murad, Yalçın Serap, Yaylacı Atılay, Ünsoy Gözde, Saylam Güleser, Akın İstemihan, Gündüz Ufuk, Korkmaz Hakan
Central Laboratory, Molecular Biology and Biotechnology R&D, Middle East Technical University, 06800, Ankara, Turkey.
Department of Otorhinolaryngology, Dışkapı Yıldırım Beyazıt Training and Research Hospital, Ankara, Turkey.
Eur Arch Otorhinolaryngol. 2015 Dec;272(12):3779-84. doi: 10.1007/s00405-014-3435-2. Epub 2014 Dec 16.
DNA repair systems are essential for normal cell function. Genetic alterations in the DNA repair genes such as X-ray repair cross-complementing group 3 (XRCC3), can cause a change in protein activity which results in cancer susceptibility. The aim of this study was to investigate the association of XRCC3 Thr241Met single nucleotide polymorphism (SNP), smoking and alcohol consumption with the risk of laryngeal cancer in Turkish population. The frequencies of Thr241Met SNP were studied in 58 laryngeal cancer cases (SSC) and 67 healthy individuals. Genomic DNA was isolated from peripheral blood samples of both controls and laryngeal cancer cases. Thr241Met SNP was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The genotype and allele frequencies of Thr241Met polymorphism were not statistically significant between the laryngeal cancer and control groups. Carrying mutant allele was not associated with the risk of laryngeal cancer. On the other hand, smoking and chronic alcohol consumption were associated with the risk of laryngeal cancer but there is no association between Thr241Met, smoking and alcohol consumption in laryngeal cancer cases. These results indicate that Thr241Met polymorphism was not associated with the development of laryngeal cancer in Turkish population. However, it should be kept in mind that the association of a polymorphism with cancer susceptibility can differ due to several factors such as cancer type, selection criteria, ethnic differences and size of the studied population.
DNA修复系统对正常细胞功能至关重要。DNA修复基因如X射线修复交叉互补基因3（XRCC3）的基因改变，可导致蛋白质活性变化，进而引发癌症易感性。本研究旨在探讨XRCC3 Thr241Met单核苷酸多态性（SNP）、吸烟和饮酒与土耳其人群喉癌风险的关联。在58例喉癌病例（SSC）和67名健康个体中研究了Thr241Met SNP的频率。从对照组和喉癌病例的外周血样本中分离基因组DNA。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对Thr241Met SNP进行基因分型。喉癌组和对照组之间Thr241Met多态性的基因型和等位基因频率无统计学意义。携带突变等位基因与喉癌风险无关。另一方面，吸烟和长期饮酒与喉癌风险相关，但在喉癌病例中Thr241Met、吸烟和饮酒之间无关联。这些结果表明，在土耳其人群中，Thr241Met多态性与喉癌的发生无关。然而，应该记住，由于癌症类型、选择标准、种族差异和研究人群规模等多种因素，多态性与癌症易感性的关联可能会有所不同。
