Atalay M A, Koc A N, Demir G, Sav H
Department of Clinical Microbiology, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
Niger J Clin Pract. 2015 Jan-Feb;18(1):52-5. doi: 10.4103/1119-3077.146979.
The Candida species, which are one of the most common causes of nosocomial bloodstream infections, present with high mortality and morbidity rates. This study aims to investigate the production of esterase, phospholipase, proteinase, and biofilm formation ability of the Candida strains isolated from the blood cultures.
Between June 2011 and July 2012, the Candida strains, which were isolated from blood cultures of a total of 50 patients, were studied. The esterase activity was analyzed in the Tween-80 agar, while phospholipase activity was studied in the egg yolk agar. The proteinase activity and biofilm formation were identified by using the petri dish method and microplate method, respectively.
Of 50 specimens obtained from individual patients, 17 (34%) were identified as C. albicans, 14 (28%) as C. glabrata, 9 (18%) as C. parapsilosis, 5 (10%) as C. krusei, 4 (8%) as C. kefyr, and 1 (2%) as C. tropicalis. The rate of proteinase, phospholipase, and esterase positivity was higher in the C. albicans isolates. Biofilm formation was the highest in the C. parapsilosis strains.
Higher rate of virulence factors in the most commonly isolated Candida species than other species indicates that these virulence factors play a crucial role in the pathogenesis.
念珠菌属是医院血流感染最常见的病因之一，其死亡率和发病率都很高。本研究旨在调查从血培养中分离出的念珠菌菌株的酯酶、磷脂酶、蛋白酶产生情况及生物膜形成能力。
在2011年6月至2012年7月期间，对从总共50例患者的血培养中分离出的念珠菌菌株进行了研究。在吐温80琼脂中分析酯酶活性，在蛋黄琼脂中研究磷脂酶活性。分别使用培养皿法和微孔板法鉴定蛋白酶活性和生物膜形成情况。
在从个体患者获得的50个标本中，17个（34%）被鉴定为白色念珠菌，14个（28%）为光滑念珠菌，9个（18%）为近平滑念珠菌，5个（10%）为克柔念珠菌，4个（8%）为凯菲念珠菌，1个（2%）为热带念珠菌。白色念珠菌分离株中蛋白酶、磷脂酶和酯酶阳性率较高。近平滑念珠菌菌株中生物膜形成率最高。
最常见分离出的念珠菌属菌株的毒力因子发生率高于其他菌株，这表明这些毒力因子在发病机制中起关键作用。
