巴西东北部三家三级医院念珠菌血症患者血培养分离出的念珠菌属的毒力因子。

Virulence factors of Candida spp. obtained from blood cultures of patients with candidemia attended at tertiary hospitals in Northeast Brazil.

机构信息

Natal City, Rio Grande do Norte, Brazil; Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte, Brazil.

São Paulo City, Laboratório Especial de Micologia, Federal University of São Paulo, São Paulo, Brazil.

出版信息

J Mycol Med. 2019 Jun;29(2):132-139. doi: 10.1016/j.mycmed.2019.02.002. Epub 2019 Mar 12.

DOI:10.1016/j.mycmed.2019.02.002

PMID:30876744

Abstract

BACKGROUND

Yeasts of the Candida genus are one of the most common causes of bloodstream infections associated with high rates of morbidity and mortality, mainly affecting immunocompromised patients. We aimed to identify yeasts obtained from blood cultures of patients interned at tertiary hospitals in Brazil.

METHODS

We evaluated some of the major virulence factors of Candida spp., including the ability to adhere to human buccal epithelial cells, biofilm formation, hemolytic and phospholipase activity.

RESULTS

We analyzed 70 isolates of Candida spp. obtained from March 2011 and March 2015. Candida spp. showed different peculiarities in terms of expression of virulence factors evaluated in vitro. C. albicans strains were more adherent to HBEC than all the other Candida species. C. tropicalis strains were considered strong biofilm producers. Strains belonging to the C. parapsilosis species complex were able to produce hemolysins, while C. glabrata was also able to lyse erythrocytes and to produce phospholipase.

CONCLUSION

These results suggest that Non-Candida albicans Candida species are also able to express virulence factors which play an important role in bloodstream infectious caused by these yeasts.

摘要

背景

念珠菌属的酵母菌是引起血流感染的最常见原因之一，这些感染与高发病率和死亡率有关，主要影响免疫功能低下的患者。我们旨在鉴定从巴西三级医院住院患者的血液培养中获得的酵母菌。

方法

我们评估了念珠菌属的一些主要毒力因子，包括黏附人类口腔上皮细胞的能力、生物膜形成、溶血和磷脂酶活性。

结果

我们分析了 2011 年 3 月至 2015 年 3 月期间获得的 70 株念珠菌属分离株。念珠菌属在体外评估的毒力因子表达方面表现出不同的特点。与所有其他念珠菌属相比，白念珠菌菌株对 HBEC 的黏附能力更强。热带念珠菌菌株被认为是强生物膜生产者。属于近平滑念珠菌种复合体的菌株能够产生溶血素，而光滑念珠菌也能够裂解红细胞并产生磷脂酶。