Kavazos Kristyn, Nataatmadja Maria, Wales Kathryn M, Hartland Elke, Williams Chloe, Russell Fraser D
Inflammation and Healing Research Cluster, School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, Queensland, Australia.
Cardiovascular Research Group, Department of Medicine, The Prince Charles Hospital, University of Queensland, Chermside, Queensland, Australia.
Heart Lung Circ. 2015 Apr;24(4):377-85. doi: 10.1016/j.hlc.2014.11.005. Epub 2014 Nov 20.
Two-day infusion of angiotensin II to apolipoprotein E-deficient (ApoE(-/-)) mice provides a model of pre-abdominal aortic aneurysm. Long chain omega-3 polyunsaturated fatty acids (n-3 PUFAs) have anti-inflammatory effects. This study examined the effect of an eight-week low or high n-3 PUFA diet in ApoE(-/-) mice on matrix metalloproteinase (MMP) expression and elastin degradation.
ApoE(-/-) mice were fed a low or high n-3 PUFA diet for eight weeks prior to two-day infusion with angiotensin II. The omega-3 index, MMP-2, MMP-9, TIMP-1, and TGF-β1 immunoreactivity, and elastin fragmentation were measured.
The omega-3 index with the low and high n-3 PUFA diet was 3.78% and 13.03%, respectively. MMP-9 immunoreactive stain intensity was lower in mice fed the high, compared to the low n-3 PUFA diet in endothelial cells (suprarenal aorta), and inflammatory cells (suprarenal and infrarenal aorta). Inflammatory cells had higher TIMP-1 and TGF-β1 stain intensity in mice fed the high, compared to the low n-3 PUFA diet (suprarenal aorta). MMP-2 immunoreactivity was unaffected by diet. A non-significant trend for reduced elastin fragmentation was observed in mice fed the high n-3 PUFA diet.
Dietary supplementation with n-3 PUFAs may have protective anti-inflammatory effects mediated through modulation of MMPs and TIMPs.
给载脂蛋白E缺陷（ApoE(-/-)）小鼠输注两天血管紧张素II可提供腹主动脉瘤前期模型。长链ω-3多不饱和脂肪酸（n-3多不饱和脂肪酸）具有抗炎作用。本研究检测了ApoE(-/-)小鼠八周低或高n-3多不饱和脂肪酸饮食对基质金属蛋白酶（MMP）表达和弹性蛋白降解的影响。
在给ApoE(-/-)小鼠输注两天血管紧张素II之前，先给予其八周低或高n-3多不饱和脂肪酸饮食。测量ω-3指数、MMP-2、MMP-9、组织金属蛋白酶抑制剂-1（TIMP-1）和转化生长因子-β1（TGF-β1）的免疫反应性以及弹性蛋白片段化情况。
低和高n-3多不饱和脂肪酸饮食组的ω-3指数分别为3.78%和13.03%。在内皮细胞（肾上腺主动脉）和炎性细胞（肾上腺和肾下腹主动脉）中，与低n-3多不饱和脂肪酸饮食组相比，高n-3多不饱和脂肪酸饮食组小鼠的MMP-9免疫反应性染色强度较低。在肾上腺主动脉中，与低n-3多不饱和脂肪酸饮食组相比，高n-3多不饱和脂肪酸饮食组小鼠的炎性细胞中TIMP-1和TGF-β1染色强度较高。饮食对MMP-2免疫反应性无影响。在高n-3多不饱和脂肪酸饮食组小鼠中观察到弹性蛋白片段化减少的趋势，但无统计学意义。
补充n-3多不饱和脂肪酸饮食可能通过调节MMP和TIMP发挥保护性抗炎作用。
