Huang Ge, Mason Susan L, Hudson J Andrew, Clerens Stefan, Plowman Jeffrey E, Hussain Malik A
Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln 7674, New Zealand.
Food Programme, Institute of Environmental Science Research, Christchurch 8540, New Zealand.
Pathogens. 2014 Dec 12;3(4):920-33. doi: 10.3390/pathogens3040920.
Listeria monocytogenes is an organism associated with a wide range of foods. It causes listeriosis, a severe illness that mainly affects people with weakened immune systems. Proteomic profiles of three different L. monocytogenes isolates were studied using 1D SDS PAGE, 2DE and mass spectrometry. The protein banding patterns generated by 1D SDS PAGE of three strains of L. monocytogenes were found to be similar. Visual observations from 2DE gel maps revealed that certain spots appeared to have intensity differences. Key differences in proteins synthesis of three strains of L. monocytogenes were found using the PDQest TM 2DE Analysis software. Comparison showed that the clinical isolate (strain SB92/844) had 53.4% and 53.9% protein profile similarity with dairy isolate (strain V7) and seafood isolate (SB92/870), respectively. The identity of selected protein spots was achieved using MALDI-TOF and ion trap mass spectrometry. It was found that certain identified proteins (i.e., a major cold shock protein and superoxide dismutase) were expressed differently between two local strains of L. monocytogenes (SB92/844, SB92/870) and one strain from overseas (V7).
单核细胞增生李斯特菌是一种与多种食物相关的微生物。它会引发李斯特菌病，这是一种主要影响免疫系统较弱人群的严重疾病。使用一维十二烷基硫酸钠聚丙烯酰胺凝胶电泳（1D SDS PAGE）、二维电泳（2DE）和质谱分析法研究了三种不同的单核细胞增生李斯特菌分离株的蛋白质组图谱。发现三种单核细胞增生李斯特菌菌株经一维十二烷基硫酸钠聚丙烯酰胺凝胶电泳产生的蛋白条带模式相似。二维电泳凝胶图谱的视觉观察显示某些斑点似乎存在强度差异。使用PDQest TM 2DE分析软件发现了三种单核细胞增生李斯特菌菌株在蛋白质合成方面的关键差异。比较表明，临床分离株（SB92/844菌株）与乳制品分离株（V7菌株）和海鲜分离株（SB92/870）的蛋白质图谱相似度分别为53.4%和53.9%。使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）和离子阱质谱分析法确定了选定蛋白斑点的身份。结果发现，在两种本地单核细胞增生李斯特菌菌株（SB92/844、SB92/870）和一种海外菌株（V7）之间，某些已鉴定的蛋白质（即一种主要的冷休克蛋白和超氧化物歧化酶）表达存在差异。
