Enteric Diseases Laboratory Branch and Bacterial Zoonoses Branch, Division of Foodborne, Bacterial, and Mycotic Diseases, National Center for Zoonotic, Vector-borne and Enteric Diseases, Coordinating Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Department of Food Science, Cornell University, Ithaca, NY 14853, USA.
Int J Syst Evol Microbiol. 2010 Jun;60(Pt 6):1280-1288. doi: 10.1099/ijs.0.014118-0. Epub 2009 Aug 10.
Four isolates (FSL S4-120(T), FSL S4-696, FSL S4-710, and FSL S4-965) of Gram-positive, motile, facultatively anaerobic, non-spore-forming bacilli that were phenotypically similar to species of the genus Listeria were isolated from soil, standing water and flowing water samples obtained from the natural environment in the Finger Lakes National Forest, New York, USA. The four isolates were closely related to one another and were determined to be the same species by whole genome DNA-DNA hybridization studies (>82 % relatedness at 55 degrees C and >76 % relatedness at 70 degrees C with 0.0-0.5 % divergence). 16S rRNA gene sequence analysis confirmed their close phylogenetic relatedness to Listeria monocytogenes and Listeria innocua and more distant relatedness to Listeria welshimeri, L. seeligeri, L. ivanovii and L. grayi. Phylogenetic analysis of partial sequences for sigB, gap, and prs showed that these isolates form a well-supported sistergroup to L. monocytogenes. The four isolates were sufficiently different from L. monocytogenes and L. innocua by DNA-DNA hybridization to warrant their designation as a new species of the genus Listeria. The four isolates yielded positive reactions in the AccuProbe test that is purported to be specific for L. monocytogenes, did not ferment L-rhamnose, were non-haemolytic on blood agar media, and did not contain a homologue of the L. monocytogenes virulence gene island. On the basis of their phenotypic characteristics and their genotypic distinctiveness from L. monocytogenes and L. innocua, the four isolates should be classified as a new species within the genus Listeria, for which the name Listeria marthii sp. nov. is proposed. The type strain of L. marthii is FSL S4-120(T) (=ATCC BAA-1595(T) =BEIR NR 9579(T) =CCUG 56148(T)). L. marthii has not been associated with human or animal disease at this time.
从美国纽约五指湖国家森林的自然环境中采集的土壤、静止水和流动水样中分离出四株革兰氏阳性、运动性、兼性厌氧、非芽孢形成的杆菌，这些杆菌表型与李斯特菌属的某些种相似。这四株菌彼此之间关系密切，通过全基因组 DNA-DNA 杂交研究确定为同一物种（55°C 时>82%的相关性，70°C 时>76%的相关性，0.0-0.5%的差异）。16S rRNA 基因序列分析证实，它们与单核细胞增生李斯特菌和无害李斯特菌密切相关，与威氏李斯特菌、塞氏李斯特菌、伊氏李斯特菌和格氏李斯特菌的关系更远。SigB、Gap 和 prs 部分序列的系统发育分析表明，这些分离株与单核细胞增生李斯特菌形成一个支持良好的姐妹群。这四个分离株与单核细胞增生李斯特菌和无害李斯特菌通过 DNA-DNA 杂交的差异足以证明它们是李斯特菌属的一个新种。这四个分离株在 AccuProbe 试验中呈阳性反应，该试验据称是单核细胞增生李斯特菌的特异性试验，它们不能发酵 L-鼠李糖，在血琼脂培养基上不溶血，也不含有单核细胞增生李斯特菌毒力基因岛的同源物。基于它们的表型特征和与单核细胞增生李斯特菌和无害李斯特菌在基因型上的明显差异，这四个分离株应被归类为李斯特菌属的一个新种，命名为马尔氏李斯特菌（Listeria marthii）。L. marthii 的模式株为 FSL S4-120(T)（=ATCC BAA-1595(T) =BEIR NR 9579(T) =CCUG 56148(T)）。到目前为止，L. marthii 尚未与人类或动物疾病有关。
