Proteomic and Biomolecular Mass Spectrometry Center, Institute of Food Science, CNR, Avellino, Italy.
J Proteomics. 2010 Sep 10;73(10):2021-30. doi: 10.1016/j.jprot.2010.06.011. Epub 2010 Jul 8.
Listeria monocytogenes, one of the major food-related pathogens, is the aetiological agent of listeriosis, a potentially life-threatening illness. It is able to survive in hostile environments and stress conditions such as those encountered in food-processing technologies (high salt concentration, wide range of pH and temperature, low water availability) and it also thrives at temperatures ranging from -0.4 to 45 °C. In this study, expression proteomics was applied to gain insight into key cellular events that allow L. monocytogenes to survive and multiply even at refrigeration temperatures. Interestingly, we observed that the adaptation processes mainly affect biochemical pathways related to protein synthesis and folding, nutrient uptake and oxidative stress. Furthermore, proteins implicated in metabolic pathways for energy production, such as glycolysis and Pta-AckA pathway, were present to a higher level in the cells grown at 4 °C. This suggests that, on the whole, cells exhibit an enhanced demand for energy to sustain cold growth. Proteomics may represent a key tool in deciphering specific mechanisms underlying cold adaptation response and, more widely, cell machinery.
单增李斯特菌是主要的食源性病原体之一，也是李斯特菌病的病原体，李斯特菌病是一种潜在的威胁生命的疾病。它能够在恶劣的环境和压力条件下生存，例如在食品加工技术中遇到的条件（高盐浓度、广泛的 pH 值和温度、低水分可用性），并且它也在从-0.4 到 45°C 的温度范围内茁壮成长。在这项研究中，表达蛋白质组学被应用于深入了解允许单核细胞增生李斯特菌即使在冷藏温度下也能生存和繁殖的关键细胞事件。有趣的是，我们观察到适应过程主要影响与蛋白质合成和折叠、营养物质摄取和氧化应激相关的生化途径。此外，在 4°C 下生长的细胞中存在更多涉及能量产生代谢途径的蛋白质，如糖酵解和 Pta-AckA 途径。这表明，总的来说，细胞为维持冷生长表现出对能量的更高需求。蛋白质组学可能是破译冷适应反应和更广泛的细胞机制背后特定机制的关键工具。
