Yu Anling, Li Yong, Ni Yingli, Yang Weibing, Yang Dongqing, Cui Zhengyong, Wang Zhenlin, Yin Yanping
National Key Laboratory of Crop Biology, Ministry of Science and Technology, Agronomy College of Shandong Agricultural University, Tai'an, Shandong, P. R. China; Agricultural Products Quality Inspection Center of Jining, Jining, Shandong, P. R. China.
National Key Laboratory of Crop Biology, Ministry of Science and Technology, Agronomy College of Shandong Agricultural University, Tai'an, Shandong, P. R. China.
PLoS One. 2014 Dec 16;9(12):e114342. doi: 10.1371/journal.pone.0114342. eCollection 2014.
Wheat starch development is a complex process and is markedly difference by changes in spikelet spatial position. The present study deals with endosperm starch granule distribution and spatial position during filling development. The study was conducted with pure starch isolated from wheat (Triticum aestivum L.), Jimai20 and Shannong1391, at 7-35 days after anthesis (DAA). The results showed that grain number, spikelet weight and grain weight per spikelet in different spatial position showed parabolic changes. Upper spikelets had highest starch and amylose content followed by basal spikelets, then middle spikelets. The paper also suggested the volume percents of B-type and A-type granule in grain of middle spikelets were remarkably higher and lower than those of basal and upper spikelets, respectively. However, no significant difference occurred in the number percents of the two type granule. The ratio of amylase to amylopectin was positively correlated with the volume proportion of 22.8-42.8 µm, but was negatively related to the volume proportion of <9.9 µm. The results indicated that the formation and distribution of starch granules were affected significantly by spikelet position, and grains at upper and basal spikelet had the potential of increasing grain weight through increasing the volume of B-type granules.
小麦淀粉的发育是一个复杂的过程，并且会因小穗空间位置的变化而显著不同。本研究探讨了灌浆发育过程中胚乳淀粉粒的分布和空间位置。该研究使用从小麦（普通小麦）济麦20和山农1391中分离出的纯淀粉，在开花后7 - 35天（DAA）进行。结果表明，不同空间位置的粒数、小穗重量和每小穗粒重呈抛物线变化。上部小穗的淀粉和直链淀粉含量最高，其次是基部小穗，然后是中部小穗。该论文还指出，中部小穗籽粒中B型和A型颗粒的体积百分比分别显著高于基部和上部小穗，而低于基部和上部小穗。然而，两种类型颗粒的数量百分比没有显著差异。淀粉酶与支链淀粉的比例与22.8 - 42.8 µm的体积比例呈正相关，但与<9.9 µm的体积比例呈负相关。结果表明，淀粉粒的形成和分布受小穗位置的显著影响，上部和基部小穗的籽粒有通过增加B型颗粒体积来增加粒重的潜力。
