芝麻菜提取物的新作用：抗血小板（抑制核因子κB）和抗血栓形成活性。
A novel role of Eruca sativa Mill. (rocket) extract: antiplatelet (NF-κB inhibition) and antithrombotic activities.
作者信息
Fuentes Eduardo, Alarcón Marcelo, Fuentes Manuel, Carrasco Gilda, Palomo Iván
机构信息
Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Interdisciplinary Excellence Research Program on Healthy Aging (PIEI-ES), Universidad de Talca, Talca 3460000, Chile.
Horticulture Department, Faculty of Agricultural Sciences, Universidad de Talca, Talca 3460000, Chile.
出版信息
Nutrients. 2014 Dec 12;6(12):5839-52. doi: 10.3390/nu6125839.
BACKGROUND
Epidemiological studies have shown the prevention of cardiovascular diseases through the regular consumption of vegetables. Eruca sativa Mill., commonly known as rocket, is a leafy vegetable that has anti-inflammatory activity. However, its antiplatelet and antithrombotic activities have not been described.
METHODS
Eruca sativa Mill. aqueous extract (0.1 to 1 mg/mL), was evaluated on human platelets: (i) P-selectin expression by flow cytometry; (ii) platelet aggregation induced by ADP, collagen and arachidonic acid; (iii) IL-1β, TGF-β1, CCL5 and thromboxane B2 release; and (iv) activation of NF-κB and PKA by western blot. Furthermore, (v) antithrombotic activity (200 mg/kg) and (vi) bleeding time in murine models were evaluated.
RESULTS
Eruca sativa Mill. aqueous extract (0.1 to 1 mg/mL) inhibited P-selectin expression and platelet aggregation induced by ADP. The release of platelet inflammatory mediators (IL-1β, TGF-β1, CCL5 and thromboxane B2) induced by ADP was inhibited by Eruca sativa Mill. aqueous extract. Furthermore, Eruca sativa Mill. aqueous extract inhibited NF-κB activation. Finally, in murine models, Eruca sativa Mill. aqueous extract showed significant antithrombotic activity and a slight effect on bleeding time.
CONCLUSION
Eruca sativa Mill. presents antiplatelet and antithrombotic activity.
背景
流行病学研究表明，经常食用蔬菜可预防心血管疾病。芝麻菜，俗称火箭菜，是一种具有抗炎活性的叶菜类蔬菜。然而，其抗血小板和抗血栓形成活性尚未见报道。
方法
对芝麻菜水提取物（0.1至1mg/mL）进行人体血小板评估：（i）通过流式细胞术检测P-选择素表达；（ii）检测ADP、胶原和花生四烯酸诱导的血小板聚集；（iii）检测白细胞介素-1β、转化生长因子-β1、趋化因子配体5和血栓素B2的释放；（iv）通过蛋白质免疫印迹法检测核因子-κB和蛋白激酶A的激活。此外，评估（v）抗血栓形成活性（200mg/kg）和（vi）小鼠模型的出血时间。
结果
芝麻菜水提取物（0.1至1mg/mL）可抑制P-选择素表达以及ADP诱导的血小板聚集。芝麻菜水提取物可抑制ADP诱导的血小板炎症介质（白细胞介素-1β、转化生长因子-β1、趋化因子配体5和血栓素B2）的释放。此外，芝麻菜水提取物可抑制核因子-κB的激活。最后，在小鼠模型中，芝麻菜水提取物显示出显著的抗血栓形成活性，对出血时间有轻微影响。
结论
芝麻菜具有抗血小板和抗血栓形成活性。
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