Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
Plant Physiol Biochem. 2015 Jan;86:181-190. doi: 10.1016/j.plaphy.2014.12.003. Epub 2014 Dec 4.
Given that limited information is available with regard to tuber dormancy release related proteome, we conducted proteome analysis of tuber dormancy release process at dormant tuber (DT), dormancy release tuber (DRT) and sprouting tuber (ST) using the iTRAQ technology. A total of 1,752 proteins were identified. Among them, a subset of 316 proteins was screened as significant up- (137) and down regulated (179) between DT vs DRT. A subset of 120 proteins experienced significant up- (40) or down-regulation (80) between DRT vs ST. The differentially expressed proteins were grouped into 11 functional categories. Proteins enriched in functional categories of major carbohydrate (CHO) metabolism, glycolysis, fermentation, amino acid metabolism, protein and transport were highly up-regulated, while functional categories of photosynthesis and RNA were down-regulated between DT vs DRT. Proteins enriched in functional groups of protein, cell wall, lipid metabolism, miscellaneous, and signaling were strongly up-regulated, while functional categories of photosynthesis, hormone metabolism and protein were down-regulated between DRT vs ST. Consistent with previous documented differentially expressed genes, most of differentially expressed proteins were also identified between DT and DRT, indicating the metabolism shift from growth suspension to growth activation as tubers dormancy breaking. The changes in protein profiles showed lower concordance with corresponding alterations in transcript levels, indicating possible transcriptional and posttranscriptional regulation. Furthermore, the possible mechanism of tuber dormancy release was discussed in relation to what was known in transcripts change and other plant models from carbohydrate metabolism, protein metabolism, stress response, redox regulation, transcription regulation, DNA metabolism, amino acid metabolism, development, signaling as well as hormone metabolism.
鉴于与块茎休眠解除相关的蛋白质组学信息有限，我们使用 iTRAQ 技术对休眠块茎（DT）、休眠解除块茎（DRT）和萌芽块茎（ST）的休眠解除过程进行了蛋白质组分析。共鉴定到 1752 种蛋白质。其中，有 316 种蛋白质在 DT 与 DRT 之间被筛选为显著上调（137 种）和下调（179 种）。在 DRT 与 ST 之间，有 120 种蛋白质经历了显著的上调（40 种）或下调（80 种）。差异表达的蛋白质被分为 11 个功能类别。在主要碳水化合物（CHO）代谢、糖酵解、发酵、氨基酸代谢、蛋白质和运输等功能类别中，富集的蛋白质高度上调，而在 DT 与 DRT 之间，光合作用和 RNA 等功能类别下调。在蛋白质、细胞壁、脂质代谢、杂项和信号等功能组中富集的蛋白质强烈上调，而在 DRT 与 ST 之间，光合作用、激素代谢和蛋白质等功能类别下调。与先前记录的差异表达基因一致，大多数差异表达蛋白也在 DT 与 DRT 之间被鉴定出来，这表明块茎休眠解除时，代谢从生长暂停转变为生长激活。蛋白质谱的变化与转录水平的相应变化一致性较低，表明可能存在转录和转录后调控。此外，还根据碳水化合物代谢、蛋白质代谢、应激反应、氧化还原调节、转录调节、DNA 代谢、氨基酸代谢、发育、信号以及激素代谢等方面的转录变化和其他植物模型，讨论了块茎休眠解除的可能机制。
