Srisapoomi Totsaporn, Ichiyanagi Tsuyoshi, Nakajima Hiromitsu, Aimi Tadanori, Boonlue Sophon
Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand,
Curr Microbiol. 2015 Apr;70(4):550-5. doi: 10.1007/s00284-014-0759-5. Epub 2014 Dec 17.
The biosynthesis of integric acid, a secondary metabolite of the wood-decay fungus Xylaria feejeensis strain 2FB-PPM08M, has been studied. Labeling experiments using [1-(13)C], [2-(13)C] and [1,2-(13)C2] acetate and L-methionine (methyl-(13)C) were separately performed with fungal culture. The labeling patterns of these metabolites indicated the same origin, and determined that integric acid was formed through the condensation of a sesquiterpene and a polyketide. These experiments showed that side chain of compounds would be synthesized by the polyketide pathway, while the ring carbon indicated the biosynthesis of compounds via the mevalonate pathway.
对腐朽木真菌费氏炭角菌菌株2FB-PPM08M的次生代谢产物整合酸的生物合成进行了研究。分别用[1-(13)C]、[2-(13)C]和[1,2-(13)C2]乙酸盐以及L-甲硫氨酸（甲基-(13)C）对真菌培养物进行标记实验。这些代谢产物的标记模式表明其来源相同，并确定整合酸是通过倍半萜和聚酮化合物的缩合形成的。这些实验表明，化合物的侧链将通过聚酮化合物途径合成，而环碳则表明化合物是通过甲羟戊酸途径生物合成的。
