Furukawa Soichi, Isomae Ryosuke, Tsuchiya Noriko, Hirayama Satoru, Yamagishi Asuka, Kobayashi Miho, Suzuki Chise, Ogihara Hirokazu, Morinaga Yasushi
a Department of Food Bioscience and Biotechnology, College of Bioresource Sciences , Nihon University , Fujisawa , Japan.
Biosci Biotechnol Biochem. 2015;79(4):681-6. doi: 10.1080/09168451.2014.991691. Epub 2014 Dec 17.
The abilities of lactic acid bacteria (LAB) to form mixed-species biofilm with Saccharomyces cerevisiae in a static co-culture were investigated out of 168 LAB stock cultures, and two Lactobacillus plantarum strains (D71 and E31) and one Leuconostoc mesenteroides strain K01 were found to form mixed-species biofilm with S. cerevisiae BY4741. SEM observation showed that there was no significant difference in morphological properties among these three mixed-species biofilms and they resembled that formed by S. cerevisiae with L. plantarum ML11-11 previously isolated from a brewing sample of Fukuyama pot vinegar. The co-aggregation assays showed that L. plantarum D71 and L. plantarum E31 could co-aggregate with S. cerevisiae similarly to L. plantarum ML11-11, while L. mesenteroides K01 had no ability to co-aggregate with yeast. The above results indicate that aggregation followed by direct cell-to-cell contact is required for mixed-species biofilm formation between these L. plantarum strains and S. cerevisiae, though some different mechanism may be involved in biofilm formation between L. mesenteroides strain and S. cerevisiae.
从168株乳酸菌库存培养物中研究了乳酸菌（LAB）与酿酒酵母在静态共培养中形成混合物种生物膜的能力，发现两株植物乳杆菌菌株（D71和E31）和一株肠膜明串珠菌菌株K01能与酿酒酵母BY4741形成混合物种生物膜。扫描电子显微镜观察表明，这三种混合物种生物膜的形态特性没有显著差异，并且它们类似于酿酒酵母与先前从福山锅醋酿造样品中分离出的植物乳杆菌ML11-11形成的生物膜。共聚集试验表明，植物乳杆菌D71和植物乳杆菌E31能够与酿酒酵母共聚集，类似于植物乳杆菌ML11-11，而肠膜明串珠菌K01没有与酵母共聚集的能力。上述结果表明，这些植物乳杆菌菌株与酿酒酵母之间形成混合物种生物膜需要通过直接细胞间接触进行聚集，尽管肠膜明串珠菌菌株与酿酒酵母之间的生物膜形成可能涉及一些不同的机制。
