柬埔寨不明原因病毒性呼吸道感染集群的多重聚合酶链反应分析
Multiplex PCR analysis of clusters of unexplained viral respiratory tract infection in Cambodia.
作者信息
Ly Nary, Tokarz Rafal, Mishra Nischay, Sameroff Stephen, Jain Komal, Rachmat Agus, An Ung Sam, Newell Steven, Harrison Dustin J, Lipkin W Ian
机构信息
U.S. Naval Medical Research Unit-2 Detachment, Phnom Penh, Cambodia.
Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York City, USA.
出版信息
Virol J. 2014 Dec 17;11:224. doi: 10.1186/s12985-014-0224-x.
BACKGROUND
Fevers of unknown origin constitute a substantial disease burden in Southeast Asia. In majority of the cases, the cause of acute febrile illness is not identified.
METHODS
We used MassTag PCR, a multiplex assay platform, to test for the presence of 15 viral respiratory agents from 85 patients with unexplained respiratory illness representing six disease clusters that occurred in Cambodia between 2009 and 2012.
RESULTS
We detected a virus in 37 (44%) of the cases. Human rhinovirus, the virus detected most frequently, was found in both children and adults. The viruses most frequently detected in children and adults, respectively, were respiratory syncytial virus and enterovirus 68. Sequence analysis indicated that two distinct clades of enterovirus 68 were circulating during this time period.
CONCLUSIONS
This is the first report of enterovirus 68 in Cambodia and contributes to the appreciation of this virus as an important respiratory pathogen.
背景
不明原因发热在东南亚构成了相当大的疾病负担。在大多数情况下，急性发热性疾病的病因无法确定。
方法
我们使用MassTag PCR（一种多重检测平台）对来自柬埔寨2009年至2012年间发生的六个疾病集群的85例不明原因呼吸道疾病患者进行15种病毒性呼吸道病原体检测。
结果
我们在37例（44%）病例中检测到病毒。人鼻病毒是最常检测到的病毒，在儿童和成人中均有发现。儿童和成人中最常检测到的病毒分别是呼吸道合胞病毒和肠道病毒68型。序列分析表明，在此期间有两种不同进化枝的肠道病毒68型在传播。
结论
这是柬埔寨关于肠道病毒68型的首次报告，有助于认识该病毒是一种重要的呼吸道病原体。
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