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一种用于研究微波对培养细胞影响的系统。

A system for studying effects of microwaves on cells in culture.

作者信息

Chen K C, Lin C I

出版信息

J Microw Power. 1978 Sep;13(3):251-6. doi: 10.1080/16070658.1978.11689102.

DOI:10.1080/16070658.1978.11689102
PMID:255162
Abstract

An improved design of the fluid-filled waveguide-exposure chamber is described for studying effects of microwave radiation on cells in vitro. The system with a micropipette sample holder may be used as a prototype to isolate the apparent nonthermal factor of microwave radiation on cells in culture from those effects resulting from cell temperature rise. This system also allows more precise calibration of incident and absorbed microwave energies. Compared with control, somatic cells of the Chinese hamster exhibited a lower rate of growth and difference in morphology after 2450 MHz microwave radiation for 20 min at a power density of 500 mW/cm2.

摘要

本文描述了一种用于研究微波辐射对体外细胞影响的改进型充液波导暴露室设计。带有微量移液器样品架的系统可作为一个原型,用于将微波辐射对培养细胞的明显非热因素与细胞温度升高所产生的影响区分开来。该系统还能更精确地校准入射和吸收的微波能量。与对照组相比,中国仓鼠的体细胞在功率密度为500 mW/cm² 的2450 MHz微波辐射20分钟后,生长速率较低且形态出现差异。

相似文献

1
A system for studying effects of microwaves on cells in culture.一种用于研究微波对培养细胞影响的系统。
J Microw Power. 1978 Sep;13(3):251-6. doi: 10.1080/16070658.1978.11689102.
2
In vitro microwave effects on human neutrophil precursor cells (CFU-C).体外微波对人中性粒细胞前体细胞(集落形成单位 - 粒细胞,CFU - C)的影响。
Bioelectromagnetics. 1981;2(3):203-15. doi: 10.1002/bem.2250020302.
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Effect of microwave radiation on cells treated with membrane-injuring substances.微波辐射对经膜损伤物质处理的细胞的影响。
Exp Pathol (Jena). 1977;13(6):296-301. doi: 10.1016/s0014-4908(77)80016-1.
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Thermal effects of 2.45 GHz microwaves on survival and viability of Chinese hamster V-79 cells.2.45吉赫兹微波对中国仓鼠V - 79细胞存活和活力的热效应
Int J Radiat Biol Relat Stud Phys Chem Med. 1987 Aug;52(2):325-35. doi: 10.1080/09553008714551781.
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Observations of Syrian hamster fetuses after exposure to 2450-MHz microwaves.
J Microw Power. 1982 Jun;17(2):107-12. doi: 10.1080/16070658.1982.11689270.
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Low power microwave radiation inhibits the proliferation of rabbit lens epithelial cells by upregulating P27Kip1 expression.低功率微波辐射通过上调P27Kip1表达抑制兔晶状体上皮细胞的增殖。
Mol Vis. 2004 Feb 25;10:138-43.
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Temperature and corticosterone relationships in microwave-exposed rats.微波辐射大鼠体温与皮质酮的关系
J Appl Physiol Respir Environ Exerc Physiol. 1978 Mar;44(3):438-45. doi: 10.1152/jappl.1978.44.3.438.
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Acute thermoregulatory responses of the immature rat to warming by low-level 2,450-MHz microwave radiation.未成熟大鼠对低水平2450兆赫微波辐射升温的急性体温调节反应。
Biol Neonate. 1989;56(1):48-56. doi: 10.1159/000242986.
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Effects of 2.45 GHz CW microwave radiation on embryofetal development in mice.2.45吉赫兹连续波微波辐射对小鼠胚胎胎儿发育的影响。
Teratology. 1981 Dec;24(3):303-14. doi: 10.1002/tera.1420240309.
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The relationship between colony-forming ability, chromosome aberrations and incidence of micronuclei in V79 Chinese hamster cells exposed to microwave radiation.暴露于微波辐射下的V79中国仓鼠细胞的集落形成能力、染色体畸变与微核发生率之间的关系。
Mutat Res. 1991 Jul;263(3):143-9. doi: 10.1016/0165-7992(91)90054-8.

引用本文的文献

1
Microwave fixation: its potential for routine techniques, histochemistry, immunocytochemistry and electron microscopy.微波固定:其在常规技术、组织化学、免疫细胞化学及电子显微镜检查中的应用潜力
Histochem J. 1984 Nov;16(11):1171-91. doi: 10.1007/BF01003442.
2
The effect of 2450 MHz microwave radiation on histamine secretion by rat peritoneal mast cells.2450兆赫微波辐射对大鼠腹膜肥大细胞组胺分泌的影响。
Cell Biophys. 1980 Jun;2(2):127-38. doi: 10.1007/BF02795839.
3
Differentiating the effects of microwave and heat on tissue proteins and their crosslinking by formaldehyde.
区分微波和热对组织蛋白质的影响及其与甲醛的交联作用。
Histochem J. 1988 Jun-Jul;20(6-7):341-6. doi: 10.1007/BF01002727.