Fuchinoue Fumi, Hirotani Yukari, Nakanishi Yoko, Yamaguchi Hiromi, Nishimaki Haruna, Noda Hiroko, Tang Xiao Yan, Iizuka Misato, Amano Sadao, Sugitani Masahiko, Nemoto Norimichi, Masuda Shinobu
Department of Pathology, Nihon University School of Medicine, Tokyo, Japan.
Pathol Int. 2015 Jan;65(1):19-26. doi: 10.1111/pin.12235. Epub 2014 Dec 16.
Apocrine carcinoma is categorized as a special type of breast carcinoma because of its specific morphological features. To clarify the characteristics of apocrine carcinoma from the point of view of the mitochondrial profile, we conducted a comparative study between apocrine and non-apocrine carcinomas. The expressions of mitochondrial related factors (PGC1α, Nrf1, Nrf2, mtTFA and COX4) were examined in a testing set of breast cancer tissue. Apocrine carcinomas showed a clear tendency towards higher mRNA expression levels of PGC1α than non-apocrine carcinomas. The expression of the selected factor, PGC1α, as well as that of p62 was further examined. The results revealed that apocrine carcinomas showed a higher immunohistochemical positivity rate for PGC1α (21.3% vs. 3.2%; P = 0.008), and that the mRNA expression level of PGC1α was significantly higher in apocrine carcinoma than in non-apocrine carcinoma (P = 0.007). The immunohistochemical positivity rate for p62 protein was also higher in apocrine carcinomas (44.7% vs. 21.0%; P = 0.015), although no significant difference in the p62 mRNA expression level was detected between the two types of carcinoma (P = 0.633). In conclusion, this study revealed that apocrine carcinoma overexpressed PGC1α contributing to mitochondrial biogenesis, and also p62 protein accumulation.
大汗腺癌因其特殊的形态学特征而被归类为一种特殊类型的乳腺癌。为了从线粒体特征的角度阐明大汗腺癌的特点，我们对大汗腺癌和非大汗腺癌进行了一项比较研究。在一组乳腺癌组织检测样本中检测了线粒体相关因子（PGC1α、Nrf1、Nrf2、mtTFA和COX4）的表达。大汗腺癌显示出PGC1α的mRNA表达水平明显高于非大汗腺癌的趋势。进一步检测了所选因子PGC1α以及p62的表达。结果显示，大汗腺癌的PGC1α免疫组化阳性率更高（21.3%对3.2%；P = 0.008），并且大汗腺癌中PGC1α的mRNA表达水平显著高于非大汗腺癌（P = 0.007）。大汗腺癌中p62蛋白的免疫组化阳性率也更高（44.7%对21.0%；P = 0.015），尽管在两种类型的癌之间未检测到p62 mRNA表达水平的显著差异（P = 0.633）。总之，本研究表明大汗腺癌中PGC1α过表达有助于线粒体生物合成，并且还存在p62蛋白积累。
