Nozaki Fumi, Hirotani Yukari, Nakanishi Yoko, Yamaguchi Hiromi, Nishimaki Haruna, Noda Hiroko, Tang Xiaoyan, Yamamoto Hisae, Suzuki Atsuko, Seki Toshimi, Masuda Shinobu
Department of Pathology, St. Luke's International Hospital, Tokyo, Japan; Department of Oncologic Pathology, Nihon University School of Medicine, Tokyo, Japan.
Division of Morphological and Functional Pathology, Department of Pathology and Microbiology, Nihon University School of Medicine , Tokyo, Japan.
Acta Histochem Cytochem. 2016 Aug 30;49(4):125-30. doi: 10.1267/ahc.16013. Epub 2016 Aug 3.
p62, also called sequestosome 1 (SQSTM1), is a multifunctional signaling molecule that affects cell proliferation. Recently, we found accumulation of p62 in apocrine carcinoma of the breast, however, the biological role of p62 expression in apocrine carcinoma still remains unclear. To investigate whether p62 might contribute to tumor cell proliferation in apocrine carcinomas, we used the MDA-MB-453 (androgen receptor-positive, HER2-type) and MFM223 (androgen receptor-positive, triple-negative type) breast cancer cell lines as models of molecular apocrine carcinoma. Both MDA-MB-453 and MFM223 showed strong and d high p62 protein expression than MCF7 cells (androgen receptor-negative, luminal A type). Knockdown of p62 resulted in significant reduction of the cell proliferative activity in both MDA-MB-453 (P<0.01) and MFM223 (P<0.05). In conclusion, p62 could contribute to cell proliferation and represent a therapeutic target in apocrine carcinoma.
p62，也称为聚集体蛋白1（SQSTM1），是一种影响细胞增殖的多功能信号分子。最近，我们发现p62在乳腺大汗腺癌中积聚，然而，p62在大汗腺癌中表达的生物学作用仍不清楚。为了研究p62是否可能促进大汗腺癌中的肿瘤细胞增殖，我们使用MDA-MB-453（雄激素受体阳性，HER2型）和MFM223（雄激素受体阳性，三阴性型）乳腺癌细胞系作为分子大汗腺癌的模型。与MCF7细胞（雄激素受体阴性，管腔A型）相比，MDA-MB-453和MFM223均显示出强烈且较高的p62蛋白表达。敲低p62导致MDA-MB-453（P<0.01）和MFM223（P<0.05）的细胞增殖活性显著降低。总之，p62可能促进细胞增殖，并代表大汗腺癌的一个治疗靶点。
