Yu Ran, Ye Zheng, Li Jing, Li Min, Bai Yu, Pan Qun-wan
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2015 Jan;31(1):49-53.
To analyse the relationship between the electrical activity changes of nucleus accumbens (NAc) shell and the drug-seeking behavior by recording NAc shell electrical activity in conditioned place preference (CPP) rats induced by morphine.
Forty SD rats were randomly divided into operation-only control group and the morphine-induced CPP group after stereotaxic electrode was buried on rats NAc shell and the latter group was used to establish the morphine CPP model(n = 20). A CPP video system combining with the technique of electrical activity wireless telemetry was used in the study. The NAc electrical activity from each group of rats was recorded by wireless telemetry respectively, which included staying in black or white chamber of video box, shuttling between black-white chambers and between white-black chambers. The electrical activity differences were analyzed by the percentage of each wave.
When the morphine-induced rats staying in black chamber, compared with the operation-only control group, the NAc shell electrical activity showed that the percentage of 0 - 10 Hz was increased(P < 0.05), meanwhile, those of 10 - 20 Hz and 30 - 40 Hz were reduced(P < 0.05, P < 0.01); when the morphine-induced rats staying in white chamber, the NAc shell electrical activity showed that the percentage of 0 - 10 Hz and 30 - 40 Hz were increased(P < 0.05 , P < 0.01) , that of 10 - 20 Hz was reduced(P < 0.05 , P < 0. 01); when the morphine-induced rats in black- white shuttling status, the NAc shell electrical activity showed that the percentage of 0 - 10 Hz was increased(P <0.05, P <0.01), that of 10- 30 Hz was reduced( P <0.05); and in the white-black shuttling status, the electrical activity showed that the percentage of 0 - 10 Hz was reduced(P <0.05), that of 10 - 30 Hz was increased(P < 0.05) ; the electrical activity was further compared between staying status and shuttling status in the morphine-induced CPP group. There was no significant difference of electrical activity between the rats in white-black shuttling status and staying in white chamber. However, when rats in black-white shuttling status, compared with staying in black chamber, the electrical activity showed that the percentage of 0 - 10 Hz and 40 - 50 Hz were increased(P < 0.05), meanwhile, those of 10 - 20 Hz and 30 - 40 Hz were reduced(P <0.05).
The electrical activity changes of NAc shell in morphine-induced CPP rats were different from those of the operation-only control group, and these changes might be associated to the rat's drug-seeking behavior.
通过记录吗啡诱导的条件性位置偏爱（CPP）大鼠伏隔核（NAc）壳区的电活动，分析其与觅药行为之间的关系。
40只SD大鼠在脑立体定位埋植电极于NAc壳区后，随机分为单纯手术对照组和吗啡诱导CPP组，后者用于建立吗啡CPP模型（n = 20）。本研究采用结合电活动无线遥测技术的CPP视频系统。分别通过无线遥测记录每组大鼠的NAc电活动，包括在视频箱黑白室停留、黑白室间穿梭以及白黑室间穿梭时的电活动。通过各波百分比分析电活动差异。
吗啡诱导大鼠在黑室时，与单纯手术对照组相比，NAc壳区电活动显示0 - 10Hz百分比增加（P < 0.05），同时10 - 20Hz和30 - 40Hz百分比降低（P < 0.05，P < 0.01）；吗啡诱导大鼠在白室时，NAc壳区电活动显示0 - 10Hz和30 - 40Hz百分比增加（P < 0.05，P < 0.01），10 - 20Hz百分比降低（P < 0.05，P < 0.01）；吗啡诱导大鼠在黑白穿梭状态时，NAc壳区电活动显示0 - 10Hz百分比增加（P < 0.05，P < 0.01），10 - 30Hz百分比降低（P < 0.05）；在白黑穿梭状态时，电活动显示0 - 10Hz百分比降低（P < 0.05），10 - 30Hz百分比增加（P < 0.05）；进一步比较吗啡诱导CPP组大鼠停留状态和穿梭状态的电活动。白黑穿梭状态大鼠与在白室停留大鼠的电活动无显著差异。然而，当大鼠处于黑白穿梭状态时，与在黑室停留相比，电活动显示0 - 10Hz和40 - 50Hz百分比增加（P < 0.05），同时10 - 20Hz和30 - 40Hz百分比降低（P < 0.05）。
吗啡诱导的CPP大鼠NAc壳区电活动变化与单纯手术对照组不同，这些变化可能与大鼠的觅药行为有关。
