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在伊朗米纳布、鲁丹和巴沙加德采用巢式PCR法检测治疗患者中无症状间日疟原虫携带者

Detection of Asymptomatic Carriers of Plasmodium vivax among Treated Patients by Nested PCR Method in Minab, Rudan and Bashagard, Iran.

作者信息

Shahbazi Abbas, Farhadi Pegah, Yerian Masoud, Bazmani Ahad, Nakhjiri Sara Khadem, Rasouli Arash, Raeisi Ahmad

机构信息

Tabriz Research Center of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran.

Dept. of Medical Parasitology and Mycology, Tabriz University of Medical Sciences, Tabriz, Iran.

出版信息

Iran J Parasitol. 2013 Oct;8(4):586-92.

PMID:25516740
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4266123/
Abstract

BACKGROUND

Plasmodium vivax is the most widespread species of Plasmodium in humans and causing about 80 million clinical cases annually. This study was undertaken to detect P. vivax in asymptomatic treated vivax malaria patients to trace latent/sub-patent malaria infection.

METHOD

The venous blood of all detected cases with P. vivax in Bashagard, Minab and Roodan Districts in Hormozgan Province from 2009 to 2010 was examined by microscopic and nested PCR methods for presence of the parasite.

RESULTS

In microscopic examination of peripheral blood smears, all samples were negative for the presence of the parasites. But, we detected two P. vivax related bands in the electrophoresis of the nested PCR products (120 bp).

CONCLUSION

Following up the malaria cases after treatment by a combination of methods, or new diagnostics such as RDTs can be included in the priorities of malaria elimination program in Iran.

摘要

背景

间日疟原虫是人类中分布最广泛的疟原虫种类,每年导致约8000万例临床病例。本研究旨在检测无症状的间日疟治愈患者中的间日疟原虫,以追踪潜在/亚临床疟疾感染。

方法

对2009年至2010年在霍尔木兹甘省巴沙加德、米纳卜和鲁丹地区所有检测出间日疟原虫的病例的静脉血,采用显微镜检查和巢式PCR方法检测寄生虫的存在情况。

结果

在外周血涂片的显微镜检查中,所有样本的寄生虫检测均为阴性。但是,我们在巢式PCR产物的电泳中检测到两条间日疟原虫相关条带(120bp)。

结论

通过多种方法对疟疾病例进行治疗后随访,或采用如快速诊断检测等新的诊断方法,可纳入伊朗疟疾消除计划的优先事项。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64e9/4266123/9d90f36aa09f/IJPA-8-586f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64e9/4266123/9d90f36aa09f/IJPA-8-586f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64e9/4266123/9d90f36aa09f/IJPA-8-586f1.jpg

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2
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Iran J Public Health. 2010;39(2):105-9. Epub 2010 Jun 30.
3
Plasmodium vivax dhfr mutations among isolates from malarious areas of Iran.伊朗疟疾流行地区分离株间的间日疟原虫二氢叶酸还原酶突变
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Malar J. 2016 May 28;15(1):299. doi: 10.1186/s12936-016-1334-2.
4
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Iran J Parasitol. 2015 Apr-Jun;10(2):197-205.
Korean J Parasitol. 2011 Jun;49(2):125-31. doi: 10.3347/kjp.2011.49.2.125. Epub 2011 Jun 14.
4
Detection of asymptomatic malaria infection among the Afghani immigrant population in Iranshahr district of southeastern Iran.伊朗东南部伊兰沙赫尔地区阿富汗移民人群中无症状疟疾感染的检测。
Bull Soc Pathol Exot. 2011 Oct;104(4):321-3. doi: 10.1007/s13149-011-0134-8. Epub 2011 Feb 9.
5
A large proportion of asymptomatic Plasmodium infections with low and sub-microscopic parasite densities in the low transmission setting of Temotu Province, Solomon Islands: challenges for malaria diagnostics in an elimination setting.在所罗门群岛的低传播环境下,在坦莫图省存在大量无症状且寄生虫密度低和亚显微的疟原虫感染:消除环境下疟疾诊断的挑战。
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