在伊朗米纳布、鲁丹和巴沙加德采用巢式PCR法检测治疗患者中无症状间日疟原虫携带者
Detection of Asymptomatic Carriers of Plasmodium vivax among Treated Patients by Nested PCR Method in Minab, Rudan and Bashagard, Iran.
作者信息
Shahbazi Abbas, Farhadi Pegah, Yerian Masoud, Bazmani Ahad, Nakhjiri Sara Khadem, Rasouli Arash, Raeisi Ahmad
机构信息
Tabriz Research Center of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran.
Dept. of Medical Parasitology and Mycology, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
Iran J Parasitol. 2013 Oct;8(4):586-92.
BACKGROUND
Plasmodium vivax is the most widespread species of Plasmodium in humans and causing about 80 million clinical cases annually. This study was undertaken to detect P. vivax in asymptomatic treated vivax malaria patients to trace latent/sub-patent malaria infection.
METHOD
The venous blood of all detected cases with P. vivax in Bashagard, Minab and Roodan Districts in Hormozgan Province from 2009 to 2010 was examined by microscopic and nested PCR methods for presence of the parasite.
RESULTS
In microscopic examination of peripheral blood smears, all samples were negative for the presence of the parasites. But, we detected two P. vivax related bands in the electrophoresis of the nested PCR products (120 bp).
CONCLUSION
Following up the malaria cases after treatment by a combination of methods, or new diagnostics such as RDTs can be included in the priorities of malaria elimination program in Iran.
背景
间日疟原虫是人类中分布最广泛的疟原虫种类，每年导致约8000万例临床病例。本研究旨在检测无症状的间日疟治愈患者中的间日疟原虫，以追踪潜在/亚临床疟疾感染。
方法
对2009年至2010年在霍尔木兹甘省巴沙加德、米纳卜和鲁丹地区所有检测出间日疟原虫的病例的静脉血，采用显微镜检查和巢式PCR方法检测寄生虫的存在情况。
结果
在外周血涂片的显微镜检查中，所有样本的寄生虫检测均为阴性。但是，我们在巢式PCR产物的电泳中检测到两条间日疟原虫相关条带（120bp）。
结论
通过多种方法对疟疾病例进行治疗后随访，或采用如快速诊断检测等新的诊断方法，可纳入伊朗疟疾消除计划的优先事项。
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