Shahbazi A, Mirhendi H, Raeisi A
Dept. of Parasitology, School of Medicine, Tabriz University of Medical Sciences, Tabriz Research Center of Infectious Diseases And Tropical Diseases, Iran.
Iran J Public Health. 2010;39(2):105-9. Epub 2010 Jun 30.
The importance of accurate diagnosis of all of major diseases cannot be underestimated and efficient laboratory testing is vital to identifying and treating life-threatening illnesses including malaria. In this study, we compared the potential of one of merozoite surface protein genes, PvMSP-3β, for detection of Plasmodium vivax in blood samples by PCR with routinely used marker, ssrRNA gene.
One hundred P. vivax microscopy-positive blood samples were simultaneously tested with two genetic markers, including PvMSP-3β gene and ssrRNA gene by PCR and nestedPCR method, respectively, and their sensitivity and specificity in detection of P. vivax was compared.
An important difference was seen in sensitivity between the 2 genetic markers, 100% in case of ssrRNA gene vs. 95% of PvMSP-3β gene. The specificity of the two markers was same (100%). Microscopic diagnoses of thick and thin blood smears was used as "golden standard" method.
Due to critical importance of accurate detection of the parasite in malarious area, the PvMSP-3β gene cannot be a suitable marker for detection of P. vivax in blood sample by PCR. More investigations are needed to find other valid markers.
准确诊断所有重大疾病的重要性不可低估，高效的实验室检测对于识别和治疗包括疟疾在内的危及生命的疾病至关重要。在本研究中，我们通过聚合酶链反应（PCR），将裂殖子表面蛋白基因之一的间日疟原虫裂殖子表面蛋白3β（PvMSP-3β）与常规使用的标记物小亚基核糖体RNA（ssrRNA）基因在检测血样中间日疟原虫的潜力进行了比较。
100份间日疟原虫显微镜检查阳性血样，分别通过PCR和巢式PCR方法，同时用两种基因标记物（包括PvMSP-3β基因和ssrRNA基因）进行检测，并比较它们检测间日疟原虫的敏感性和特异性。
两种基因标记物在敏感性上存在重要差异，ssrRNA基因的敏感性为100%，而PvMSP-3β基因的敏感性为95%。两种标记物的特异性相同（均为100%）。厚血膜和薄血膜的显微镜诊断被用作“金标准”方法。
由于在疟疾流行地区准确检测疟原虫至关重要，PvMSP-3β基因不能作为通过PCR检测血样中间日疟原虫的合适标记物。需要更多研究来寻找其他有效的标记物。
