Bai Guang-Yi, Zhou Feng, Hui Yu, Xu Yong-De, Lei Hong-En, Pu Jin-Xian, Xin Zhong-Cheng
Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.
Department of Urology, First Affiliated Hospital of Soochow University, Suzhou 215006, China.
Int J Mol Sci. 2014 Dec 15;15(12):23294-306. doi: 10.3390/ijms151223294.
Diabetic erectile dysfunction is associated with penile dorsal nerve bundle neuropathy in the corpus cavernosum and the mechanism is not well understood. We investigated the neuropathy changes in the corpus cavernosum of rats with streptozotocin-induced diabetes and the effects of Icariside II (ICA II) on improving neuropathy. Thirty-six 8-week-old Sprague-Dawley rats were randomly distributed into normal control group, diabetic group and ICA-II treated group. Diabetes was induced by a one-time intraperitoneal injection of streptozotocin (60 mg/kg). Three days later, the diabetic rats were randomly divided into 2 groups including a saline treated placebo group and an ICA II-treated group (5 mg/kg/day, by intragastric administration daily). Twelve weeks later, erectile function was measured by cavernous nerve electrostimulation with real time intracorporal pressure assessment. The penis was harvested for the histological examination (immunofluorescence and immunohistochemical staining) and transmission electron microscopy detecting. Diabetic animals exhibited a decreased density of dorsal nerve bundle in penis. The neurofilament of the dorsal nerve bundle was fragmented in the diabetic rats. There was a decreased expression of nNOS and NGF in the diabetic group. The ICA II group had higher density of dorsal nerve bundle, higher expression of NGF and nNOS in the penis. The pathological change of major pelvic nerve ganglion (including the microstructure by transmission electron microscope and the neurite outgrowth length of major pelvic nerve ganglion tissue cultured in vitro) was greatly attenuated in the ICA II-treated group (p < 0.01). ICA II treatment attenuates the diabetes-related impairment of corpus cavernosum and major pelvic ganglion neuropathy in rats with Streptozotocin-Induced Diabetes.
糖尿病性勃起功能障碍与海绵体阴茎背神经束神经病变有关，但其机制尚不完全清楚。我们研究了链脲佐菌素诱导的糖尿病大鼠海绵体的神经病变变化以及淫羊藿次苷II（ICA II）对改善神经病变的作用。将36只8周龄的Sprague-Dawley大鼠随机分为正常对照组、糖尿病组和ICA-II治疗组。通过一次性腹腔注射链脲佐菌素（60 mg/kg）诱导糖尿病。三天后，将糖尿病大鼠随机分为2组，包括生理盐水治疗的安慰剂组和ICA II治疗组（5 mg/kg/天，每日灌胃给药）。12周后，通过海绵体神经电刺激并实时评估海绵体内压力来测量勃起功能。摘取阴茎进行组织学检查（免疫荧光和免疫组织化学染色）以及透射电子显微镜检测。糖尿病动物阴茎背神经束密度降低。糖尿病大鼠背神经束的神经丝断裂。糖尿病组nNOS和NGF表达降低。ICA II组阴茎背神经束密度更高，NGF和nNOS表达更高。在ICA II治疗组中，主要盆腔神经节的病理变化（包括透射电子显微镜下的微观结构以及体外培养的主要盆腔神经节组织的神经突生长长度）明显减轻（p < 0.01）。ICA II治疗可减轻链脲佐菌素诱导的糖尿病大鼠海绵体与糖尿病相关的损伤以及主要盆腔神经节神经病变。
