Scholle R R, Robb S M, Robb F T, Woods D R
Department of Microbiology, University of Cape Town, South Africa.
Gene. 1989 Aug 1;80(1):49-56. doi: 10.1016/0378-1119(89)90249-7.
The nucleotide sequence of a 2.119-kb DNA fragment containing the Vibrio alginolyticus sucrase gene (scrB) was determined. The complete sequence (484 aa residues) of the sucrase was deduced and homology was detected between the sucrase enzymes from V. alginolyticus and the Gram-positive bacteria Bacillus subtilis and Streptococcus mutans. In Escherichia coli cells the cloned V. alginolyticus sucrase is translocated to the periplasm. Transposon phoA mutagenesis experiments strongly suggested that V. alginolyticus sucrase in E. coli is not exported across the cytoplasmic membrane by means of a typical signal sequence.
测定了包含溶藻弧菌蔗糖酶基因（scrB）的一个2.119 kb DNA片段的核苷酸序列。推导了蔗糖酶的完整序列（484个氨基酸残基），并检测到溶藻弧菌与革兰氏阳性菌枯草芽孢杆菌和变形链球菌的蔗糖酶之间存在同源性。在大肠杆菌细胞中，克隆的溶藻弧菌蔗糖酶被转运到周质空间。转座子phoA诱变实验有力地表明，大肠杆菌中的溶藻弧菌蔗糖酶不是通过典型的信号序列穿过细胞质膜输出的。
