Huang Ri-Long, Han Shu, Qin Li-Hong, Sun Zhong-Ren
The First Affiliated Hospital of Anhui College of Chinese Medicine, Hefei 230031, China.
Zhen Ci Yan Jiu. 2012 Jun;37(3):224-8.
To observe the effect of electroacupuncture (EA) preconditioning of "Neiguan" (PC 6) on myocardial enzyme levels in acute recurrent myocardial ischemia rabbits so as to study its mechanisms underlying protection of ischemic myocardium.
A total of 40 healthy rabbits were randomized into normal control, myocardial ischemia (MI) model, EA preconditioning (EA-P), recurrent MI (RMI), EA-P+ RMI groups. MI model was duplicated by auricular intravenous administration of Posterior Pituitary Injection (PPI, 0.3 mL/kg). EA (2 Hz/70 Hz, 2-4 mA) was applied to bilateral PC 6 for 30 min, once daily for 7 d before MI. The contents of serum aspartate aminotransferase (AST), isoenzymes of creatine kinase (CK-MB), lactate dehydrogenase (LDH), creatine kinase (CK), alpha-hydroxybutyrate dehydrogenase (alpha-HBD) were detected by automatic biochemistry analyzer.
Following administration of PPI, ECG-ST(II), serum AST, CK-MB, LDH, CK and alpha-HBD levels were increased significantly in the MI and RMI groups (P<0.01). Compared with the MI model group, ECG-ST(II), serum AST, CK-MB, LDH, CK and alpha-HBD levels were down-regulated considerably in the EA-P group (P<0.01), and ECG-ST(II), serum AST, CK-MB, LDH, CK and alpha-HBD levels were obviously lower in the EA-P+ RMI group than in the RMI group (P<0.01). No significant differences were found between the MI and RMI groups, and between EA-P and EA-P + RMI groups in the above-mentioned 6 indexes (P>0.05).
Electroacupuncture preconditioning has a remarkable effect in the prevention of ischemic myocardial injury of both acute and recurrent myocardial ischemia in the rabbit, which is closely associated with its functions in lowering serum AST, CK-MB, LDH, CK and alpha-HBD contents.
观察电针“内关”（PC 6）预处理对急性复发性心肌缺血家兔心肌酶水平的影响，以探讨其对缺血心肌保护作用的机制。
将40只健康家兔随机分为正常对照组、心肌缺血（MI）模型组、电针预处理（EA-P）组、复发性心肌缺血（RMI）组、EA-P + RMI组。采用耳缘静脉注射垂体后叶素（PPI，0.3 mL/kg）复制MI模型。在MI前，每天1次，连续7 d，将电针（2 Hz/70 Hz，2 - 4 mA）施加于双侧PC 6 30 min。用自动生化分析仪检测血清天冬氨酸氨基转移酶（AST）、肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（LDH）、肌酸激酶（CK）、α-羟丁酸脱氢酶（α-HBD）含量。
注射PPI后，MI组和RMI组心电图ST（II）段、血清AST、CK-MB、LDH、CK及α-HBD水平显著升高（P<0.01）。与MI模型组比较，EA-P组心电图ST（II）段、血清AST、CK-MB、LDH、CK及α-HBD水平明显下调（P<0.01）；EA-P + RMI组心电图ST（II）段、血清AST、CK-MB、LDH、CK及α-HBD水平明显低于RMI组（P<0.01）。上述6项指标在MI组与RMI组之间、EA-P组与EA-P + RMI组之间比较，差异无统计学意义（P>0.05）。
电针预处理对家兔急性和复发性心肌缺血性心肌损伤均有显著的预防作用，这与其降低血清AST、CK-MB、LDH、CK及α-HBD含量的作用密切相关。
