Boondit Jitrada, Pipatsatitpong Duangnate, Mungthin Mathirut, Taamasri Paanjit, Tan-ariya Peerapan, Naaglor Tawee, Leelayoova Saovanee
J Med Assoc Thai. 2014 Feb;97 Suppl 2:S52-9.
Blastocystis infection is one of the most common intestinal protozoan infections reported in Thai population of all age groups for which epidemiological information is important to understand patterns of transmission for developing methods ofprevention and control for each specific group. The authors aimed to study prevalence, incidence and riskfactors associated with Blastocystis infection in orphans and childcare workers. Additionally, subtypes ofBlastocystis were identified
A retrospective cohort study of Blastocystis sp. was conducted in orphans aged less than 5 years and their childcare workers at Babies' Home, Nonthaburi Province, Thailand. A base line survey was conducted in December 2009 and afollow-up survey was conducted in April 2010. A total of 336 and 331 stool samples were collected. Blastocystis infection was examined using short-term in vitro cultivation in Jones's medium supplemented with 10% fetal calf serum. To analyze subtypes ofBlastocystis sp., PCR-RFLP of the small subunit ribosomal RNA gene was performed.
Theprevalence ofBlastocystis infection in December 2009 and April 2010 were 8.1% and 13.3%, respectively The inlcidence rate ofBlastocystis infection was 1.6/100 person-months. Subtype analysis ofBlastocystis sp. in December 2009 and in April 2010 showed that subtype 3 was the most predominant (76% and 76%), followed by subtype 1 (16% and 20%), and unidentified subtype (8% and 4%), respectively. Subtype 3 is of human origin, thus person-to-person transmission is considered a major route ofBlastocystis infection in this population.
Person-to-person transmission of Blastocystis infection in orphans living in the same house had been proposed, thus the prevalence and incidence of Blastocystis infection could be used to reflect the hygienic condition in the orphanage. Infection prevention and control practice can be effectively implemented.
芽囊原虫感染是泰国各年龄组人群中报告的最常见的肠道原生动物感染之一，了解其流行病学信息对于掌握传播模式以制定针对每个特定群体的预防和控制方法至关重要。作者旨在研究孤儿和儿童保育员中芽囊原虫感染的患病率、发病率及相关危险因素。此外，还对芽囊原虫的亚型进行了鉴定。
在泰国暖武里府婴儿之家对5岁以下孤儿及其儿童保育员进行了一项关于芽囊原虫的回顾性队列研究。2009年12月进行了基线调查，2010年4月进行了随访调查。共收集了336份和331份粪便样本。使用添加10%胎牛血清的琼斯培养基进行短期体外培养来检测芽囊原虫感染。为分析芽囊原虫的亚型，对小亚基核糖体RNA基因进行了PCR-RFLP分析。
2009年12月和2010年4月芽囊原虫感染的患病率分别为8.1%和13.3%。芽囊原虫感染的发病率为1.6/100人月。2009年12月和2010年4月芽囊原虫的亚型分析表明，亚型3最为主要（分别为76%和76%），其次是亚型1（分别为16%和20%），以及未鉴定的亚型（分别为8%和4%）。亚型3源自人类，因此人与人之间的传播被认为是该人群中芽囊原虫感染的主要途径。
已提出生活在同一房屋中的孤儿之间存在芽囊原虫感染的人际传播，因此芽囊原虫感染的患病率和发病率可用于反映孤儿院的卫生状况。可有效实施感染预防和控制措施。
