Srichaipon Nittaya, Nuchprayoon Surang, Charuchaibovorn Sarit, Sukkapan Pattadon, Sanprasert Vivornpun
Lymphatic Filariasis and Tropical Medicine Research Unit, Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Pathogens. 2019 Mar 21;8(1):38. doi: 10.3390/pathogens8010038.
spp. is one of the most common protozoa of humans and animals worldwide. The genetic diversity of spp. might be associated with a wide range of symptoms. However, the prevalence of each subtype is different in each country. Until now, there is no standard method for subtyping of spp. We developed a sequential restriction fragment length polymorphism (RFLP) analysis for the rapid differentiation of human subtypes. A large-scale study was also conducted to determine the subtype distribution of spp. in Thailand. Stool samples were collected from 1025 school-age students in four regions of Thailand. infections were identified by direct smear, formalin ethyl-acetate concentration technique (FECT), Boeck and Drbohlav's Locke-Egg-Serum (LES) medium culture, and polymerase chain reaction (PCR) of small-subunit ribosomal DNA (SSU rDNA). Subtypes of spp. were determined by RFLP. Phylogenetic tree of partial SSU rDNA sequences of spp. was constructed using the Maximum Likelihood (ML) method. Out of 1025 students, 416 (40.6%) were positive for spp. Using two steps of RFLP reactions, we could determine subtype one⁻three among these students. Subtype 3 was the most common subtype (58.72%) in Thai students, followed by subtype 1 (31.2%), and subtype 2 (10.1%). subtype 3 was the most prevalent in all regions of Thailand. The subtype distribution of spp. in Thailand was different from other countries.
[具体物种名称]是全球人类和动物中最常见的原生动物之一。[具体物种名称]的遗传多样性可能与多种症状相关。然而，每个亚型在每个国家的流行率都不同。到目前为止，尚无用于[具体物种名称]亚型分类的标准方法。我们开发了一种序列限制性片段长度多态性（RFLP）分析方法，用于快速区分人类[具体物种名称]亚型。还进行了一项大规模研究，以确定泰国[具体物种名称]的亚型分布。从泰国四个地区的1025名学龄学生中采集粪便样本。通过直接涂片、福尔马林-乙酸乙酯浓缩技术（FECT）、伯克和德博赫拉夫氏洛克-鸡蛋-血清（LES）培养基培养以及小亚基核糖体DNA（SSU rDNA）的聚合酶链反应（PCR）来鉴定[具体物种名称]感染。通过RFLP确定[具体物种名称]的亚型。使用最大似然（ML）方法构建了[具体物种名称]部分SSU rDNA序列的系统发育树。在1025名学生中，416名（40.6%）[具体物种名称]检测呈阳性。通过两步RFLP反应，我们可以确定这些学生中的亚型一至三。亚型3是泰国学生中最常见的亚型（58.72%），其次是亚型1（31.2%）和亚型2（10.1%）。[具体物种名称]亚型3在泰国所有地区最为普遍。泰国[具体物种名称]的亚型分布与其他国家不同。
