Ireland M E, Jacks L A
Department of Anatomy/Cell Biology, Wayne State University, School of Medicine, Detroit, Michigan.
Invest Ophthalmol Vis Sci. 1989 Oct;30(10):2190-4.
Crude plasma membranes were prepared from chick lens epithelial and fiber cells and assayed for specific binding of the beta-adrenergic antagonist dihydroalprenolol. Both membranes specifically bound the ligand, with the epithelial membranes exhibiting a greater number of higher affinity sites. This is the first demonstration of lens beta-adrenergic receptors using this type of assay and the first direct demonstration of beta-adrenergic receptors in lens fiber cells.
从鸡晶状体上皮细胞和纤维细胞制备粗制质膜，并检测β-肾上腺素能拮抗剂二氢心得舒的特异性结合。两种质膜都能特异性结合配体，其中上皮细胞质膜表现出更多高亲和力位点。这是首次使用此类检测方法证明晶状体β-肾上腺素能受体，也是首次直接证明晶状体纤维细胞中存在β-肾上腺素能受体。
