间接分光光度法测定标记化合物中的BIDA、DISIDA、DTPA和MDP。

Indirect spectrophotometric determination of BIDA, DISIDA, DTPA and MDP in labelled compounds.

作者信息

van der Walt T N, Coetzee P P, Fourie P J

机构信息

Department of Chemistry, Rand Afrikaans University, Johannesburg, Republic of South Africa.

出版信息

Int J Rad Appl Instrum A. 1989;40(6):525-9. doi: 10.1016/0883-2889(89)90139-1.

DOI:10.1016/0883-2889(89)90139-1

PMID:2551849

Abstract

N-(4-(n-butyl)-acetanilide)iminodiacetic acid (BIDA), N-(2,6-diisopropylacetanilide)iminodiacetic acid (DISIDA), diethylenetriaminepentaacetic acid (DTPA) and methylene diphosphonic acid (MDP) are used in labelling kits. The contents of BIDA, DISIDA or MDP of the 99mTc-labelled compounds can be determined (indirectly) spectrophotometrically with copper, eriochrome cyaanine R (ECC) and dodecylethyldimethylammonium bromide (DEDA) in a sodium barbital buffered system at pH 8.5. The calibration curves obey Beer's Law from 0 to 40 micrograms/25 mL for BIDA and DISIDA, 0 to 60 micrograms/25 mL for DTPA and 0 to 100 micrograms/10 mL for MDP.

摘要

N-（4-（正丁基）-乙酰苯胺基）亚氨基二乙酸（BIDA）、N-（2,6-二异丙基乙酰苯胺基）亚氨基二乙酸（DISIDA）、二亚乙基三胺五乙酸（DTPA）和亚甲基二膦酸（MDP）用于标记试剂盒。在pH 8.5的巴比妥钠缓冲体系中，99mTc标记化合物中的BIDA、DISIDA或MDP含量可以用铜、铬菁R（ECC）和十二烷基乙基二甲基溴化铵（DEDA）通过分光光度法（间接）测定。BIDA和DISIDA的校准曲线在0至40微克/25毫升范围内符合比尔定律，DTPA在0至60微克/25毫升范围内，MDP在0至100微克/10毫升范围内。