al-Abdulla I H, Mellor G W, Childerstone M S, Sidki A M, Smith D S
Department of Chemical Pathology, St. Bartholomew's Hospital, London, U.K.
J Immunol Methods. 1989 Sep 1;122(2):253-8. doi: 10.1016/0022-1759(89)90271-8.
The periodate and 1,1'-carbonyldiimidazole activation methods were compared with the cyanogen bromide procedure for coupling antibodies to magnetisable cellulose/iron oxide solid-phase particles. Fluoroimmunoassays for quinine, primaquine, normetanephrine and cannabinoids were employed to assess the binding properties of such coupled solid phases. The cyanogen bromide and 1,1'-carbonyldiimidazole methods gave similar products in most cases, while the specific binding capacity of periodate coupled particles was between two and five times lower. Nevertheless, comparable standard curves could be obtained with solid phase coupled by each method. The periodate and 1,1'-carbonyldiimidazole methods are acceptable alternatives, notably for laboratories lacking the facility to handle the toxic cyanogen bromide.
将高碘酸盐和1,1'-羰基二咪唑活化方法与溴化氰法进行比较，以将抗体偶联到可磁化的纤维素/氧化铁固相颗粒上。采用针对奎宁、伯氨喹、去甲变肾上腺素和大麻素的荧光免疫测定法来评估此类偶联固相的结合特性。在大多数情况下，溴化氰法和1,1'-羰基二咪唑法得到的产物相似，而高碘酸盐偶联颗粒的特异性结合能力则低两到五倍。然而，每种方法偶联的固相都能获得可比的标准曲线。高碘酸盐法和1,1'-羰基二咪唑法是可接受的替代方法，特别是对于缺乏处理有毒溴化氰设施的实验室而言。
