一种使用与可磁化颗粒偶联的抗体对结合型鹅去氧胆酸进行的直接荧光免疫测定法。

A direct fluoroimmunoassay for conjugated chenodeoxycholic acid using antibody coupling to magnetisable particles.

作者信息

Shridi F A, Chitranukroh A, Pourfarzaneh M, Billing B H, Ekeke G

出版信息

Ann Clin Biochem. 1980 Jul;17(4):188-91. doi: 10.1177/000456328001700406.

Abstract

A novel solid phase fluoroimmunoassay for conjugated chenodeoxycholic acid has been developed employing an antiserum coupled to magnetisable cellulose particles and a chenodeoxycholyl glycinefluorescein thiocarbamyl ethylene diamine conjugate as the label. The data obtained from serum samples from 25 patients correlated closely (r = 0.99) with those obtained by radioimmunoassay. The assay is rapid (30 minutes), simple, and ideal for routine clinical purposes.

摘要

一种用于结合型鹅去氧胆酸的新型固相荧光免疫分析法已经开发出来，该方法采用与可磁化纤维素颗粒偶联的抗血清以及鹅去氧胆酰甘氨酸荧光素硫代氨基甲酰乙二胺结合物作为标记物。从25名患者的血清样本中获得的数据与通过放射免疫分析法获得的数据密切相关（r = 0.99）。该分析方法快速（30分钟）、简单，非常适合常规临床应用。