Ekeke G I, Landon J, Edwards C R, White G W, Shridi F
Clin Chim Acta. 1981 Jan 8;109(1):31-7. doi: 10.1016/0009-8981(81)90133-9.
A rapid separation fluoroimmunoassay for serum or plasma levels of total oestriol in pregnancy was established, based on the use of fluorescein-labelled oestriol and sheep anti-oestriol serum covalently linked to magnetisable particles. Equilibrium was attained within 10 min, and the fluorescence of the bound fraction of labelled ligand was quantitated fluorimetrically after elution from the magnetisable particles. Results for pregnancy serum samples correlated well with an established radioimmunoassay technique and the sensitivity, precision and accuracy were appropriate for clinical use. Advantages of this system as compared with radioimmunoassay include the speed and simplicity of end-point detection, prolonged shelf-life of the labelled reactant and absence of any health hazard. The separation step enabled the removal of any endogenous fluorophores or other interfering factors present in biological samples.
建立了一种用于测定妊娠血清或血浆中总雌三醇水平的快速分离荧光免疫分析法，该方法基于使用与可磁化颗粒共价连接的荧光素标记雌三醇和羊抗雌三醇血清。10分钟内即可达到平衡，从可磁化颗粒上洗脱后，通过荧光法对标记配体结合部分的荧光进行定量。妊娠血清样本的检测结果与既定的放射免疫分析技术相关性良好，其灵敏度、精密度和准确性适用于临床应用。与放射免疫分析相比，该系统的优点包括终点检测速度快、操作简单，标记反应物的保质期延长，且不存在任何健康危害。分离步骤能够去除生物样品中存在的任何内源性荧光团或其他干扰因素。
