Li Juan, Bian Wei-He, Wan Juan, Zhou Jing, Lin Yan, Wang Ji-Rong, Wang Zhao-Xia, Shen Qun, Wang Ke-Ming
Department of Oncology, Second Affiliated Hospital of Nanjing Medical University, Nanjing, China E-mail :
Asian Pac J Cancer Prev. 2014;15(22):9997-10001. doi: 10.7314/apjcp.2014.15.22.9997.
Curdione, one of the major components of Curcuma zedoaria, has been reported to possess various biological activities. It thus might be a candidate anti-flammatory and cancer chemopreventive agent. However, the precise molecular mechanisms of action of curdione on cancer cells are still unclear. In this study, we investigated the effect of curdione on breast cancer.
Xenograft nude mice were used to detect the effect of curdione on breast cancer in vivo; we also tested the effect of curdione on breast cancer in vitro by MTT, Flow cytometry, JC-I assay, and western blot.
Firstly, we found that curdione significantly suppressed tumor growth in a xenograft nude mouse breast tumor model in a dose-dependent manner. In addition, curdione treatment inhibited cell proliferation and induced cell apoptosis. Moreover, after curdione treatment, increase of impaired mitochondrial membrane potential occurred in a concentration dependent manner. Furthermore, the expression of apoptosis-related proteins including cleaved caspase-3, caspase-9 and Bax was increased in curdione treatment groups, while the expression of the anti-apoptotic Bcl-2 was decreased. Inhibitors of caspase-3 were used to confirm that curdione induced apoptosis.
Overall, our observations first suggested that curdione inhibited the proliferation of breast cancer cells by inducing apoptosis. These results might provide some molecular basis for the anti-cancer activity of curdione.
莪术二酮是莪术的主要成分之一，据报道具有多种生物活性。因此，它可能是一种潜在的抗炎和癌症化学预防剂。然而，莪术二酮对癌细胞作用的确切分子机制仍不清楚。在本研究中，我们调查了莪术二酮对乳腺癌的影响。
采用异种移植裸鼠检测莪术二酮对乳腺癌的体内作用；我们还通过MTT、流式细胞术、JC-I检测和蛋白质免疫印迹法测试了莪术二酮对乳腺癌的体外作用。
首先，我们发现莪术二酮在异种移植裸鼠乳腺肿瘤模型中以剂量依赖的方式显著抑制肿瘤生长。此外，莪术二酮处理抑制细胞增殖并诱导细胞凋亡。而且，莪术二酮处理后，线粒体膜电位损伤的增加呈浓度依赖性。此外，在莪术二酮处理组中，包括裂解的半胱天冬酶-3、半胱天冬酶-9和Bax在内的凋亡相关蛋白的表达增加，而抗凋亡蛋白Bcl-2的表达降低。使用半胱天冬酶-３抑制剂来证实莪术二酮诱导细胞凋亡。
总体而言，我们的观察结果首次表明莪术二酮通过诱导细胞凋亡抑制乳腺癌细胞的增殖。这些结果可能为莪术二酮的抗癌活性提供一些分子基础。
