Won Kyung Hwa, Kook MooChang, Yi Tae-Hoo
Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University, Yongin, Gyeonggi, 446-701, Republic of Korea.
Antonie Van Leeuwenhoek. 2015 Feb;107(2):565-73. doi: 10.1007/s10482-014-0352-8. Epub 2014 Dec 18.
An ivory white, rod-shaped, non-motile, non-spore forming, Gram-staining-negative, and aerobic bacterium designated THG-G118T, was isolated from a soil sample collected in a bamboo plantation in Seoul, Republic of Korea. The 16S rRNA gene sequence analysis revealed a clear affiliation of strain THG-G118T with the genus Pedobacter. Strain THG-G118T was found to show the closest phylogenetic relationship with Pedobacter metabolipauper WB2.3-71T with 16S rRNA gene sequence similarity of 97.3 %. The major cellular fatty acids of strain THG-G118T were identified as C16:0, C18:0, iso-C15:0 and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), and the predominant menaquinone as menaquinone MK-7. The major polar lipids were found to be phosphatidylethanolamine (PE), unidentified phospholipid and unidentified aminophospholipids. Strain THG-G118T was found to contain ceramide phosphorylethanolamine 2 (CerPE-2) and dihydrosphingosine as the major ceramide. The G+C content of the genomic DNA was found to be 41.7 mol%. DNA-DNA hybridization experiments revealed a low level of DNA-DNA relatedness (<42.5 %) between strain THG-G118T and its closest relatives. The phenotypic, chemotaxonomic and phylogenetic data showed that strain THG-G118T could be clearly distinguished from the members of the genus Pedobacter. Based on the results of a polyphasic study, a new species, Pedobacter bambusae sp. nov., is proposed. The type strain is THG-G118T (=KACC 17544T = JCM 19364T).
从韩国首尔一个竹林采集的土壤样本中分离出一株象牙白色、杆状、无运动性、不形成芽孢、革兰氏染色阴性的需氧细菌，命名为THG-G118T。16S rRNA基因序列分析表明，THG-G118T菌株与Pedobacter属有明确的亲缘关系。发现THG-G118T菌株与Pedobacter metabolipauper WB2.3-71T的系统发育关系最为密切，16S rRNA基因序列相似性为97.3%。THG-G118T菌株的主要细胞脂肪酸被鉴定为C16:0、C18:0、异-C15:0和总和特征3（C16:1 ω7c和/或C16:1 ω6c），主要甲基萘醌为甲基萘醌MK-7。主要极性脂类被发现是磷脂酰乙醇胺（PE）、未鉴定的磷脂和未鉴定的氨基磷脂。发现THG-G118T菌株含有神经酰胺磷酸乙醇胺2（CerPE-2）和二氢鞘氨醇作为主要神经酰胺。基因组DNA的G+C含量为41.7 mol%。DNA-DNA杂交实验表明，THG-G118T菌株与其最亲近的亲缘菌株之间的DNA-DNA相关性较低（<42.5%）。表型、化学分类和系统发育数据表明，THG-G118T菌株可与Pedobacter属的成员明显区分开来。基于多相研究的结果，提出了一个新物种，即竹生Pedobacter菌（Pedobacter bambusae sp. nov.）。模式菌株为THG-G118T（=KACC 17544T = JCM 19364T）。
