Ngo Hien T T, Son Heung-Min, Park Sang-Yong, Kim Ki-Young, Yi Tae-Hoo
Antonie Van Leeuwenhoek. 2014 May;105(5):961-70. doi: 10.1007/s10482-014-0154-z.
A Gram-stain negative, strictly aerobic, motile by gliding, rod-shaped and yellow pigmented strain THG-G12T was isolated from soil of a bamboo field in Seoul, Republic of Korea. Strain THG-G12T was observed to grow well at 20–28 °C and pH 7.0–7.5 in the absence of NaCl on nutrient agar. Based on 16S rRNA gene sequence comparisons, strain THG-G12T was found to be most closely related to Pedobacter ginsengisoli Gsoil 104T (97.5 % sequence similarity), Pedobacter steynii WB2.3-45T (97.4 %), Pedobacter metabolipauper WB2.3-71T (97.2 %), Pedobacter nyackensis NWG-II14T (97.2 %), Pedobacter caeni LMG 22862T (97.1 %) and Pedobacter duraquae WB2.1-25T (97.0 %), but DNA relatedness between strain THG-G12T and its phylogenetically closest neighbours was below 9.5 %. The G+C content of the genomic DNA was determined to be 39.9 mol%. The only isoprenoid quinone detected in strain THG-G12T was menaquinone-7 (MK-7). The major component in the polyamine pattern was sym-homospermidine. The major polar lipids were found to be phosphatidylethanolamine, unidentified phosphoglycolipids, unidentified aminophosphoglycolipids, unidentified aminolipids and unidentified lipids. Strain THG-G12T showed the presence of two ceramide phosphorylethanolamines (CerPE-2′ and CerPE-2″), dihydrosphingosines and an unidentified ceramide as the major ceramide. The major fatty acids were identified as summed feature 3 (as defined by the MIDI system; C16:1 ω7c and/or C16:1 ω6c) and iso-C15:0. These data support the affiliation of strain THG-G12T to the genus Pedobacter. The results of physiological and biochemical tests enabled strain THG-G12T to be differentiated genotypically and phenotypically from the recognized species of the genus Pedobacter. Therefore, the novel isolate represents a novel species, for which the name Pedobacter seoulensis sp. nov. is proposed, with THG-G12T as the type strain (=KACC 17529T =JCM 19363T).
从韩国首尔一个竹林土壤中分离出一株革兰氏染色阴性、严格需氧、靠滑动运动、杆状且产黄色色素的菌株THG-G12T。观察到菌株THG-G12T在营养琼脂上、无氯化钠条件下、20–28 °C和pH 7.0–7.5时生长良好。基于16S rRNA基因序列比较，发现菌株THG-G12T与参地慢生根瘤菌Gsoil 104T（序列相似性97.5%）、施氏慢生根瘤菌WB2.3-45T（97.4%）、贫代谢慢生根瘤菌WB2.3-71T（97.2%）、尼亚克慢生根瘤菌NWG-II14T（97.2%）、食泥慢生根瘤菌LMG 22862T（97.1%）和硬水慢生根瘤菌WB2.1-25T（97.0%）关系最密切，但菌株THG-G12T与其系统发育关系最密切的近缘菌之间的DNA相关性低于9.5%。基因组DNA的G+C含量测定为39.9 mol%。在菌株THG-G12T中检测到的唯一类异戊二烯醌是甲基萘醌-7（MK-7）。多胺模式中的主要成分是共生高亚精胺。主要极性脂类被发现是磷脂酰乙醇胺、未鉴定的磷酸糖脂、未鉴定的氨基磷酸糖脂、未鉴定的氨基脂类和未鉴定的脂类。菌株THG-G12T显示存在两种神经酰胺磷酸乙醇胺（CerPE-2′和CerPE-2″）、二氢鞘氨醇和一种未鉴定的神经酰胺作为主要神经酰胺。主要脂肪酸被鉴定为总和特征3（如MIDI系统所定义；C16:1 ω7c和/或C16:1 ω6c）和异-C15:0。这些数据支持菌株THG-G12T属于慢生根瘤菌属。生理和生化测试结果使菌株THG-G12T能够在基因型和表型上与慢生根瘤菌属已确认的物种区分开来。因此，该新分离株代表一个新物种，提议将其命名为首尔慢生根瘤菌（Pedobacter seoulensis sp. nov.），以THG-G12T作为模式菌株（=KACC 17529T =JCM 19363T）。
