Department of Marine Biotechnology, Anyang University, Incheon 417-833, Republic of Korea.
Department of Oriental Medicinal Material & Processing College of Life Science, Kyung Hee University, Yongin, Kyunggi 446-701, Republic of Korea.
Int J Syst Evol Microbiol. 2014 May;64(Pt 5):1789-1794. doi: 10.1099/ijs.0.058024-0. Epub 2014 Feb 26.
A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacterial strain, THG-DR3T, was isolated from soil of a pine grove in Jeju island, Republic of Korea. Strain THG-DR3T grew optimally at 20-25 °C, at pH 7.0-8.0 and in R2A broth without added NaCl. 16S rRNA gene sequence analysis indicated that strain THG-DR3T belongs to the genus Pedobacter. The closest relatives based on 16S rRNA gene sequence similarity were Pedobacter aquatilis AR107T (98.0%), Pedobacter alluvionis NWER-II11T (97.7%), Pedobacter borealis G-1T (97.7%) and Pedobacter ginsengiterrae DCY49T (97.4%). The G+C content of the genomic DNA of strain THG-DR3T was 47.5 mol%. Levels of DNA-DNA relatedness between strain THG-DR3T and its closest phylogenetic neighbours were below 44.7%. The presence of menaquinone 7 and of iso-C15:0 and summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c) as the major fatty acids supported the affiliation of strain THG-DR3T to the genus Pedobacter. The polar lipids of strain THG-DR3T consisted of phosphatidylethanolamine, three unidentified aminophospholipids, five unidentified aminolipids and three unidentified lipids. The results of physiological and biochemical tests allowed the differentiation of strain THG-DR3T from recognized species of the genus Pedobacter. Strain THG-DR3T (=KACC 17172T=JCM 18824T) is proposed as the type strain of a novel species of the genus Pedobacter, Pedobacter jejuensis sp. nov. An emended description of the genus Pedobacter is also proposed.
一株革兰氏阴性、严格需氧、不运动、杆状的细菌菌株 THG-DR3T 是从韩国济州岛松林的土壤中分离出来的。THG-DR3T 菌株在 20-25°C、pH7.0-8.0 和 R2A 肉汤中生长最佳，无需添加 NaCl。16S rRNA 基因序列分析表明，THG-DR3T 菌株属于 Pedobacter 属。根据 16S rRNA 基因序列相似性，最接近的亲缘关系是 Pedobacter aquatilis AR107T（98.0%）、 Pedobacter alluvionis NWER-II11T（97.7%）、 Pedobacter borealis G-1T（97.7%）和 Pedobacter ginsengiterrae DCY49T（97.4%）。THG-DR3T 菌株基因组 DNA 的 G+C 含量为 47.5mol%。THG-DR3T 菌株与其最接近的系统发育邻居之间的 DNA-DNA 同源性水平低于 44.7%。存在menaquinone 7 和 iso-C15:0 和总和特征 3（包含 C16:1ω7c 和/或 C16:1ω6c）作为主要脂肪酸，支持 THG-DR3T 菌株属于 Pedobacter 属。THG-DR3T 菌株的极性脂由磷脂酰乙醇胺、三种未鉴定的胺磷脂、五种未鉴定的胺脂和三种未鉴定的脂组成。生理生化试验的结果使 THG-DR3T 菌株与 Pedobacter 属的公认种区分开来。THG-DR3T 菌株（=KACC 17172T=JCM 18824T）被提议为 Pedobacter 属的一个新种的模式菌株，即 Pedobacter jejuensis sp. nov.。还提出了对 Pedobacter 属的修订描述。
