Herrera Sheryl L, Palmer Vanessa L, Whittaker Heather, Smith Blair Cardigan, Kim Annie, Schellenberg Angela E, Thiessen Jonathan D, Buist Richard, Del Bigio Marc R, Martin Melanie
Physics and Astronomy, University of Manitoba, Winnipeg, Manitoba, Canada.
Biomedical Engineering Program, University of Manitoba, Winnipeg, Manitoba, Canada.
Magn Reson Insights. 2014 Nov 9;7:23-31. doi: 10.4137/MRI.S19750. eCollection 2014.
Optic chiasm lesions in myelin oligodendrocyte glycoprotein (MOG)-experimental autoimmune encephalomyelitis (EAE) mice were characterized using magnetic resonance imaging (MRI) and validated using electron microscopy (EM). MR images were collected from 3 days after induction to remission, approximately 20 days after induction. Hematoxylin and eosin, solochrome cyanin-stained sections, and EM images were obtained from the optic chiasms of some mice approximately 4 days after disease onset when their scores were thought to be the highest. T2-weighted imaging and apparent diffusion coefficient map hyperintensities corresponded to abnormalities in the optic chiasms of EAE mice. Mixed inflammation was concentrated at the lateral surface. Degeneration of oligodendrocytes, myelin, and early axonal damage were also apparent. A marked increase in chiasm thickness was observed. T2-weighted and diffusion-weighted MRI can detect abnormalities in the optic chiasms of MOG-EAE mice. MRI is an important method in the study of this model toward understanding optic neuritis.
利用磁共振成像（MRI）对髓鞘少突胶质细胞糖蛋白（MOG）-实验性自身免疫性脑脊髓炎（EAE）小鼠的视交叉病变进行表征，并通过电子显微镜（EM）进行验证。从诱导后3天至缓解期（诱导后约20天）收集MR图像。苏木精和伊红染色、单铬花青染色切片以及EM图像是在疾病发作后约4天从一些小鼠的视交叉获得的，此时它们的评分被认为是最高的。T2加权成像和表观扩散系数图高信号对应于EAE小鼠视交叉的异常。混合性炎症集中在外侧表面。少突胶质细胞、髓鞘变性以及早期轴突损伤也很明显。观察到视交叉厚度显著增加。T2加权和扩散加权MRI可以检测MOG-EAE小鼠视交叉的异常。MRI是研究该模型以了解视神经炎的重要方法。
