Lin Shih-Yi, Hsu Wu-Huei, Lin Cheng-Chieh, Chen Chao-Jung
Institute of Clinical Medical Science, China Medical University College of Medicine, 404 Taichung, Taiwan ; Department of Internal Medicine, China Medical University Hospital, 404 Taichung, Taiwan ; Division of Nephrology and Kidney Institute, China Medical University Hospital, 404 Taichung, Taiwan.
Institute of Clinical Medical Science, China Medical University College of Medicine, 404 Taichung, Taiwan ; Department of Internal Medicine, China Medical University Hospital, 404 Taichung, Taiwan ; Division of Pulmonary and Critical Care Medicine, China Medical University Hospital and China Medical University, 404 Taichung, Taiwan.
Biomedicine (Taipei). 2014;4(4):25. doi: 10.7603/s40681-014-0025-y. Epub 2014 Nov 13.
Mass spectrometry (MS) is currently the most promising tool for studying proteomics to investigate largescale proteins in a specific proteome. Emerging MS-based proteomics is widely applied to decipher complex proteome for discovering potential biomarkers. Given its growing usage in clinical medicine for biomarker discovery to predict, diagnose and confer prognosis, MS-based proteomics can benefit study of personalized medicine. In this review we introduce some fundamental MS theory and MS-based quantitative proteomic approaches as well as several representative clinical MS-based proteomics issues in Chest Medicine, Gerontology, and Nephrology.
质谱（MS）是目前研究蛋白质组学以调查特定蛋白质组中大规模蛋白质最具前景的工具。新兴的基于质谱的蛋白质组学被广泛应用于解读复杂蛋白质组以发现潜在生物标志物。鉴于其在临床医学中用于发现生物标志物以进行预测、诊断和判断预后的应用越来越广泛，基于质谱的蛋白质组学可有助于个性化医学的研究。在本综述中，我们介绍了一些基本的质谱理论、基于质谱的定量蛋白质组学方法以及胸部医学、老年医学和肾脏病学中几个具有代表性的基于临床质谱的蛋白质组学问题。
