人类皮肤和烧伤后瘢痕中的透明质酸：与胶原蛋白及其他蛋白质关联的证明

Human skin and post-burn scar hyaluronan: demonstration of the association with collagen and other proteins.

作者信息

Burd D A, Siebert J W, Ehrlich H P, Garg H G

机构信息

Department of Pathology, Harvard Medical School, Boston, MA.

出版信息

Matrix. 1989 Aug;9(4):322-7. doi: 10.1016/s0934-8832(89)80008-3.

DOI:10.1016/s0934-8832(89)80008-3

PMID:2552269

Abstract

Hyaluronan (HA) extracted from tissues has been demonstrated to have an enhancing effect on the process of wound healing; the question arises whether this effect is due to the HA or to associated collagen and other proteins. In this study, HA has been extracted from human skin and scar tissue under dissociative conditions and isolated by DEAE-cellulose chromatography followed by CsCl gradient and Sepharose CL-6B chromatography. This highly purified HA was found to contain between 4 and 28% protein, with collagen constituting 5% of the total protein. A functional association between HA and a collagen protein complex is proposed.

摘要

从组织中提取的透明质酸（HA）已被证明对伤口愈合过程有促进作用；问题在于这种作用是由于HA本身，还是与其相关的胶原蛋白和其他蛋白质。在本研究中，HA是在解离条件下从人皮肤和瘢痕组织中提取的，并通过DEAE - 纤维素色谱法，随后进行CsCl梯度和琼脂糖CL - 6B色谱法进行分离。发现这种高度纯化的HA含有4%至28%的蛋白质，其中胶原蛋白占总蛋白的5%。有人提出HA与胶原蛋白蛋白复合物之间存在功能关联。