Kagan J, Tuveson R W, Gong H H
Department of Chemistry, University of Illinois, Chicago 60680.
Mutat Res. 1989 Oct;216(5):231-42. doi: 10.1016/0165-1161(89)90048-4.
In vitro, the photodynamic compound benzo[a]pyrene (BAP) generates singlet oxygen efficiently when irradiated in organic solvents. It also photogenerates superoxide anion radical in water and can act as a photoreducing agent in the absence of oxygen. In vivo, the hemolysis of human erythrocytes, the inactivation of Escherichia coli cells representing a series of strains differing in excision repair and catalase proficiency, and the inactivation of Haemophilus influenzae transforming DNA activity were used to characterize the phototoxicity of BAP in the presence of near-UV light (290-400 nm). The results are consistent with BAP behaving as a photosensitizer that generates both superoxide and singlet oxygen, and that damages chiefly membranes. DNA does not seem to be a major target in the phototoxic reactions investigated.
在体外，光动力化合物苯并[a]芘（BAP）在有机溶剂中受辐照时能高效产生单线态氧。它在水中也能光生成超氧阴离子自由基，并且在无氧条件下可作为光还原剂。在体内，利用人红细胞的溶血、代表一系列在切除修复和过氧化氢酶活性方面存在差异的菌株的大肠杆菌细胞的失活，以及流感嗜血杆菌转化DNA活性的失活，来表征在近紫外光（290 - 400 nm）存在下BAP的光毒性。结果表明，BAP表现为一种能同时产生超氧和单线态氧的光敏剂，主要损伤细胞膜。在所研究的光毒性反应中，DNA似乎不是主要靶点。
