Tuveson R W, Kagan J, Shaw M A, Moresco G M, von Behne E M, Pu H, Bazin M, Santus R
Department of Microbiology, University of Illinois, Urbana 61801.
Environ Mol Mutagen. 1987;10(3):245-61. doi: 10.1002/em.2850100304.
Fluoranthene, a non-carcinogenic polycyclic aromatic hydrocarbon, inactivates Escherichia coli cells in the presence of near-ultraviolet light (NUV; 300-400 nm). E coli cells carrying defects in the uvrA6 or katF genes are sensitized to inactivation by the simultaneous treatment with fluoranthene and NUV, suggesting that DNA is a target and that hydrogen peroxide is generated. Haemophilus influenzae transforming DNA can be inactivated by the simultaneous treatment with fluoranthene and NUV confirming DNA as a target. Using the photooxidation of imidazole and histidine as probes, fluoranthene was found to generate singlet oxygen in organic and aqueous media. In water, it participated in electron transfer reactions, reducing nitro blue tetrazolium as well as ferricytochrome C. This reduction took place both in the presence of air, where superoxide anion was formed, and under argon. Simultaneous treatment with fluoranthene and NUV was incapable of inducing histidine-independent mutations. Simultaneous treatment with fluoranthene and NUV was incapable of inducing the uvrA gene product as evidenced by the absence of the induction of beta-galactosidase in an E coli operon fusion strain [uvrA215::Mud(Ap,lac)].
荧蒽是一种非致癌性多环芳烃，在近紫外光（NUV；300 - 400纳米）存在的情况下可使大肠杆菌细胞失活。携带uvrA6或katF基因缺陷的大肠杆菌细胞在用荧蒽和NUV同时处理时对失活敏感，这表明DNA是一个靶点且会产生过氧化氢。流感嗜血杆菌转化DNA在用荧蒽和NUV同时处理时可被灭活，证实DNA是一个靶点。以咪唑和组氨酸的光氧化作为探针，发现荧蒽在有机介质和水性介质中均能产生单线态氧。在水中，它参与电子转移反应，可还原硝基蓝四氮唑以及铁细胞色素C。这种还原反应在有空气（会形成超氧阴离子）存在的情况下以及在氩气环境下均会发生。用荧蒽和NUV同时处理无法诱导不依赖组氨酸的突变。在用荧蒽和NUV同时处理时无法诱导uvrA基因产物，这在大肠杆菌操纵子融合菌株[uvrA215::Mud(Ap,lac)]中β - 半乳糖苷酶未被诱导这一现象中得到证实。
