Spiegelberg Linda, Braks Joanna A M, Groeneveldt Lisanne C, Djasim Urville M, van der Wal Karel G H, Wolvius Eppo B
Department of Oral and Maxillofacial Surgery (Head: Eppo B. Wolvius, MD, DMD, PhD), Erasmus University Medical Center, 3000 CA, Rotterdam, The Netherlands.
Department of Oral and Maxillofacial Surgery (Head: Eppo B. Wolvius, MD, DMD, PhD), Erasmus University Medical Center, 3000 CA, Rotterdam, The Netherlands.
J Craniomaxillofac Surg. 2015 Mar;43(2):214-9. doi: 10.1016/j.jcms.2014.11.008. Epub 2014 Nov 18.
Radiation therapy (RT) as part head and neck cancer treatment often leads to irradiation of surrounding normal tissue, such as mandibular bone. A reduced reparative capacity of the bone can lead to osteoradionecrosis (ORN). Hyperbaric oxygen therapy (HBOT) is used to treat ORN, based on its potential to raise the oxygen tension in tissues. However, prevention of radiation-induced damage is of great interest. Our purpose was to investigate whether HBOT could prevent radiation-induced damage to murine mandibles.
Twenty-eight mice were irradiated in the head and neck region with a single dose (15 Gy) and half of them were subsequently subjected to HBOT. Another 14 mice did not receive any treatment and served as controls. Ten and 24 weeks after RT, mandibles were harvested and analysed histologically and by microcomputed tomography (micro-CT).
Micro-CT analysis showed a reduction in relative bone volume by RT, which was partly recovered by HBOT. Trabecular thickness and separation were also positively influenced by HBOT. Morphologically, HBOT suppressed the osteoclast number, indicating decreased resorption, and decreased the amount of lacunae devoid of osteocytes, indicating increased bone viability.
HBOT was able to partly reduce radiation-induced effects on microarchitectural parameters, resorption, and bone viability in mouse mandibles. HBOT could therefore potentially play a role in the prevention of radiation-induced damage to human mandibular bone.
放射治疗（RT）作为头颈部癌治疗的一部分，常常会导致周围正常组织如下颌骨受到照射。骨修复能力降低会导致放射性骨坏死（ORN）。高压氧治疗（HBOT）基于其提高组织中氧张力的潜力被用于治疗ORN。然而，预防辐射诱导的损伤备受关注。我们的目的是研究HBOT是否能预防辐射对小鼠下颌骨的损伤。
28只小鼠接受单次剂量（15 Gy）的头颈部照射，其中一半随后接受HBOT治疗。另外14只小鼠未接受任何治疗作为对照。放疗后10周和24周，采集下颌骨并进行组织学分析和显微计算机断层扫描（micro-CT）分析。
Micro-CT分析显示放疗导致相对骨体积减少，而HBOT部分恢复了该体积。小梁厚度和间距也受到HBOT的积极影响。形态学上，HBOT抑制破骨细胞数量，表明吸收减少，减少了无骨细胞的腔隙数量，表明骨活力增加。
HBOT能够部分减轻辐射对小鼠下颌骨微观结构参数、吸收和骨活力的影响。因此，HBOT可能在预防辐射对人类下颌骨的损伤中发挥作用。
