Schumacher M, Coirini H, McEwen B S
Laboratory of Neuroendocrinology, Rockefeller University, New York, N.Y.
Neuroendocrinology. 1989 Sep;50(3):315-20. doi: 10.1159/000125239.
The regulation of 3H-muscimol binding to high-affinity GABAa receptors by estradiol (E) and by progesterone (P) was studied within discrete brain regions using in vitro quantitative autoradiography. Treatment of ovariectomized and adrenalectomized female rats with E resulted in a decrease of muscimol binding only in specific estrogen-sensitive brain regions like the ventromedial nuclei (VMN) of the hypothalamus, the arcuate nucleus (ARC), the medial amygdala and the midbrain central grey (MCG). When administered alone, P had no effect. However, in estrogen-primed females, P increased muscimol binding in both VMN and MCG to levels seen in control animals. Thus, E and P exert opposite effects on the GABAa receptor within these two nuclei. As both hormones facilitate female reproductive behavior as well as the release of luteinizing hormone, present results suggest that E and P affect muscimol binding by different mechanisms.
采用体外定量放射自显影技术，研究了雌二醇（E）和孕酮（P）对离散脑区中3H-蝇蕈醇与高亲和力γ-氨基丁酸A型（GABAa）受体结合的调节作用。用E处理去卵巢和肾上腺切除的雌性大鼠，仅在下丘脑腹内侧核（VMN）、弓状核（ARC）、内侧杏仁核和中脑中央灰质（MCG）等特定雌激素敏感脑区，蝇蕈醇结合减少。单独给予P时无作用。然而，在雌激素预处理的雌性大鼠中，P使VMN和MCG中的蝇蕈醇结合增加至对照动物的水平。因此，E和P对这两个核内的GABAa受体发挥相反作用。由于这两种激素都促进雌性生殖行为以及促黄体生成素的释放，目前的结果表明E和P通过不同机制影响蝇蕈醇结合。
