Oh J S, Paik D I, Christensen R, Akoto-Amanfu E, Kim K, Park N H
UCLA School of Dentistry.
Oral Surg Oral Med Oral Pathol. 1989 Oct;68(4):428-35. doi: 10.1016/0030-4220(89)90141-2.
We studied the effects of herpes simplex virus type 1 (HSV-1) inoculation and topical 7,12-dimethylbenz[a]anthracene (DMBA) application, alone or in combination, on the carcinogenesis and on the amplification and expression of various cellular proto-oncogenes in hamster buccal pouch tissue. Topical DMBA treatment produced tumor formation in pouches, but HSV-1 inoculation, alone caused no neoplastic changes. In pouch tissues receiving both DMBA application and HSV-1 inoculation, the development of initial leukoplakia and tumor has hastened and enhanced in comparison with those receiving DMBA alone. Topical DMBA application to pouch tissue induced an amplification and an increase in the expression of cellular erb-B-1 (c-erb-B-1) proto-oncogene in the epithelial tissue, whereas repeated infection with HSV-1 alone did not. Topical DMBA combined with HSV-1 inoculation, however, resulted in greater amplification and expression of c-erb-B-1 proto-oncogene in the pouch epithelial tissue compared to the DMBA alone. These data indicate that HSV-1 inoculation significantly increases the carcinogenic activity of DMBA, in part, by probably enhancing DMBA-induced amplification and expression of c-erb-B-1 proto-oncogene in hamster buccal pouch tissue.
我们研究了单纯疱疹病毒1型（HSV-1）接种和局部应用7,12-二甲基苯并[a]蒽（DMBA）单独或联合使用对仓鼠颊囊组织致癌作用以及各种细胞原癌基因扩增和表达的影响。局部应用DMBA治疗可使颊囊产生肿瘤，但单独接种HSV-1未引起肿瘤性改变。与仅接受DMBA治疗的颊囊组织相比，同时接受DMBA应用和HSV-1接种的颊囊组织中，初期白斑和肿瘤的发生加快且加重。局部应用DMBA可诱导上皮组织中细胞erb-B-1（c-erb-B-1）原癌基因的扩增和表达增加，而单独反复感染HSV-1则不会。然而，与单独使用DMBA相比，局部应用DMBA联合HSV-1接种导致颊囊上皮组织中c-erb-B-1原癌基因的扩增和表达更强。这些数据表明，HSV-1接种可能部分通过增强DMBA诱导的仓鼠颊囊组织中c-erb-B-1原癌基因的扩增和表达，从而显著增加DMBA的致癌活性。
