Min B M, Kim K, Cherrick H M, Park N H
UCLA School of Dentistry, Center for Health Sciences 90024.
In Vitro Cell Dev Biol. 1991 Feb;27A(2):128-36. doi: 10.1007/BF02630998.
Three squamous carcinoma cell lines HBPC-1, HBPC-2, and HBPC-3 were established from hamster buccal pouch tumors induced by topical 7,12-dimethylbenz(a)anthracene (DMBA) treatment alone, topical DMBA treatment in conjunction with type 1 herpes simplex virus (HSV-1) inoculation, and topical DMBA application in combination with type 2 HSV (HSV-2) inoculation, respectively. The cells were epithelial in morphology, had a doubling time of approximately 18 h, and required bovine serum for optimal growth. They demonstrated an in vitro anchorage-independent growth and produced squamous cell carcinomas when transplanted into normal hamster pouch submucosa. The carcinoma cell lines equally expressed cellular hst, src, abl, and raf proto-oncogenes that were not expressed in the normal hamster pouch epithelial cells. An equal amount of fos gene expression was noticed in the normal pouch epithelial cells, HBPC-1 and HBPC-3, but the HBPC-2 expressed less fos poly(A+)RNA than the other cell lines. The myc proto-oncogene was also expressed both in the normal pouch epithelial cells and in the cancer cell lines. However, the size and number of expressed myc poly(A+)RNA in the normal cells and cancer cell lines differed. Although the normal cells and HBPC-1 expressed a single myc transcript, 1.7-kilobase (kb) and 2.3-kb, respectively, both HBPC-2 and HBPC-3 expressed two myc poly(A+)RNAs, 1.7-kb and 2.3-kb.
三种鳞状癌细胞系HBPC - 1、HBPC - 2和HBPC - 3分别从仅经局部7,12 - 二甲基苯并(a)蒽（DMBA）处理、局部DMBA处理联合1型单纯疱疹病毒（HSV - 1）接种、局部DMBA应用联合2型HSV（HSV - 2）接种诱导的仓鼠颊囊肿瘤中建立。这些细胞在形态上呈上皮样，倍增时间约为18小时，最佳生长需要牛血清。它们在体外表现出不依赖贴壁的生长特性，移植到正常仓鼠颊囊黏膜下层时会产生鳞状细胞癌。这些癌细胞系均表达细胞hst、src、abl和raf原癌基因，而在正常仓鼠颊囊上皮细胞中不表达。在正常颊囊上皮细胞、HBPC - 1和HBPC - 3中观察到等量的fos基因表达，但HBPC - 2表达的fos poly(A+)RNA比其他细胞系少。myc原癌基因在正常颊囊上皮细胞和癌细胞系中也均有表达。然而，正常细胞和癌细胞系中表达的myc poly(A+)RNA的大小和数量有所不同。尽管正常细胞和HBPC - 1分别表达单一的myc转录本，大小为1.7千碱基（kb）和2.3 kb，但HBPC - 2和HBPC - 3均表达两种myc poly(A+)RNA，分别为1.7 kb和2.3 kb。
