对来自不同宿主的鱼类病原菌鲑鱼立克次氏体的两个分离株进行比较基因组分析，揭示了与毒力相关的分泌系统的主要差异。
Comparative Genome Analysis of Two Isolates of the Fish Pathogen Piscirickettsia salmonis from Different Hosts Reveals Major Differences in Virulence-Associated Secretion Systems.
作者信息
Bohle Harry, Henríquez Patricio, Grothusen Horst, Navas Esteban, Sandoval Alvaro, Bustamante Fernando, Bustos Patricio, Mancilla Marcos
机构信息
Laboratorio de Diagnóstico y Biotecnología, ADL Diagnostic Chile Ltda., Puerto Montt, Chile.
Laboratorio de Diagnóstico y Biotecnología, ADL Diagnostic Chile Ltda., Puerto Montt, Chile
出版信息
Genome Announc. 2014 Dec 18;2(6):e01219-14. doi: 10.1128/genomeA.01219-14.
Abstract
Outbreaks caused by Piscirickettsia salmonis are one of the major threats to the sustainability of the Chilean salmon industry. We report here the annotated draft genomes of two P. salmonis isolates recovered from different salmonid species. A comparative analysis showed that the number of virulence-associated secretion systems constitutes a main genomic difference.
摘要
由鲑鱼立克次氏体引发的疫情是智利鲑鱼养殖业可持续发展面临的主要威胁之一。我们在此报告从不同鲑科鱼类物种中分离出的两株鲑鱼立克次氏体的注释基因组草图。比较分析表明，与毒力相关的分泌系统数量是主要的基因组差异所在。
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2
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J Fish Dis. 2015 Apr;38(4):415-8. doi: 10.1111/jfd.12255. Epub 2014 Jun 10.
3
Efficient de novo assembly of highly heterozygous genomes from whole-genome shotgun short reads.利用全基因组鸟枪法短读长数据对高杂合基因组进行高效的从头组装。
Genome Res. 2014 Aug;24(8):1384-95. doi: 10.1101/gr.170720.113. Epub 2014 Apr 22.
4
RepARK--de novo creation of repeat libraries from whole-genome NGS reads.RepARK——从头创建来自全基因组 NGS 读取的重复文库。
Nucleic Acids Res. 2014 May;42(9):e80. doi: 10.1093/nar/gku210. Epub 2014 Mar 14.
5
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6
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7
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