Jia Hangxing, Liang Tong, Wang Zhaoning, He Zhaoren, Liu Yang, Yang Lei, Zeng Yan, Liu Shaopeng, Tang Linyi, Wang Jianbo, Chen Yu, Xie Zhixiong
College of Life Sciences, Wuhan University , Wuhan, Hubei 430072, China.
ACS Synth Biol. 2014 Dec 19;3(12):1007-10. doi: 10.1021/sb500029n.
Accurately controlling expression of target genes between several designed levels is essential for low-noise gene network and dynamic range of gene expression. However, such manipulations have been hard to achieve due to technical limitations. Based on tandem promoters and CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated) system, we constructed a multistage regulator that could stably regulate the expression of the reporter gene on three levels, with more than 2-fold difference between each of them. Our findings provide novel insights into constructing a more powerful gene regulation system.
在几个设计水平之间精确控制靶基因的表达对于低噪声基因网络和基因表达的动态范围至关重要。然而，由于技术限制，这种操作一直难以实现。基于串联启动子和CRISPR（成簇规律间隔短回文重复序列）/Cas（CRISPR相关）系统，我们构建了一种多级调节器，它可以在三个水平上稳定调节报告基因的表达，每个水平之间的差异超过2倍。我们的发现为构建更强大的基因调控系统提供了新的见解。
