Kim Jin-Young, Fluri David A, Kelm Jens M, Hierlemann Andreas, Frey Olivier
Eidgenössische Technische Hochschule Zürich, Department of Biosystems Science and Engineering, Bio Engineering Laboratory, Basel, Switzerland
InSphero AG, Schlieren, Switzerland.
J Lab Autom. 2015 Jun;20(3):274-82. doi: 10.1177/2211068214564056. Epub 2014 Dec 18.
In this article, we present a microfluidic platform, compatible with conventional 96-well formats, that enables facile and parallelized culturing and testing of spherical microtissues in a standard incubator. The platform can accommodate multiple microtissues (up to 66) of different cell types, formed externally by using the hanging-drop method, and enables microtissue interconnection through microfluidic channels for continuous media perfusion or dosage of substances. The platform contains 11 separate channels, and each channel has six tissue compartments. Primary rat liver tissues were cultured over 8 days, and multiple tumor tissues (HCT116) were exposed to various concentrations of 5-fluorouracil for platform characterization.
在本文中，我们展示了一种与传统96孔板格式兼容的微流控平台，该平台能够在标准培养箱中轻松且并行地培养和测试球形微组织。该平台可容纳通过悬滴法在外部形成的多种不同细胞类型的微组织（多达66个），并通过微流控通道实现微组织互连，以进行连续的培养基灌注或物质给药。该平台包含11个独立通道，每个通道有六个组织隔室。原代大鼠肝脏组织培养了8天，多种肿瘤组织（HCT116）暴露于不同浓度的5-氟尿嘧啶以对该平台进行表征。
