Misun Patrick M, Birchler Axel K, Lang Moritz, Hierlemann Andreas, Frey Olivier
Bio Engineering Laboratory, Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.
IST Austria, Klosterneuburg, Austria.
Methods Mol Biol. 2018;1771:183-202. doi: 10.1007/978-1-4939-7792-5_15.
The hanging-drop network (HDN) is a technology platform based on a completely open microfluidic network at the bottom of an inverted, surface-patterned substrate. The platform is predominantly used for the formation, culturing, and interaction of self-assembled spherical microtissues (spheroids) under precisely controlled flow conditions. Here, we describe design, fabrication, and operation of microfluidic hanging-drop networks.
悬滴网络（HDN）是一种基于倒置的、表面有图案的基板底部完全开放的微流控网络的技术平台。该平台主要用于在精确控制的流动条件下自组装球形微组织（球体）的形成、培养和相互作用。在此，我们描述微流控悬滴网络的设计、制造和操作。
