Babin A, Saciat C, Teixeira M, Troussard J-P, Motreuil S, Moreau J, Moret Y
Équipe Écologie Évolutive, UMR CNRS 6282 Biogéosciences, Université de Bourgogne, Dijon, France.
Équipe Écologie Évolutive, UMR CNRS 6282 Biogéosciences, Université de Bourgogne, Dijon, France.
Dev Comp Immunol. 2015 Apr;49(2):278-81. doi: 10.1016/j.dci.2014.12.007. Epub 2014 Dec 15.
The release of reactive oxygen and nitrogen species (ROS and RNS) during the inflammatory response generates damages to host tissues, referred to as immunopathology, and is an important factor in ecological immunology. The integrated antioxidant system, comprising endogenous antioxidant enzymes (e.g. superoxide dismutase SOD, and catalase CAT) and dietary antioxidants (e.g. carotenoids), helps to cope with immune-mediated oxidative stress. Crustaceans store large amounts of dietary carotenoids for yet unclear reasons. While being immunostimulants and antioxidants, the interaction of these pigments with antioxidant enzymes remains unclear. Here, we tested the interaction between dietary supplementation with carotenoids and immune challenge on immune defences and the activity of the antioxidant enzymes SOD and CAT, in the amphipod crustacean Gammarus pulex. Dietary supplementation increased the concentrations of circulating carotenoids and haemocytes in the haemolymph, while the immune response induced the consumption of circulating carotenoids and a drop of haemocyte density. Interestingly, supplemented gammarids exhibited down-regulated SOD activity but high CAT activity compared to control ones. Our study reveals specific interactions of dietary carotenoids with endogenous antioxidant enzymes, and further underlines the potential importance of carotenoids in the evolution of immunity and/or of antioxidant mechanisms in crustaceans.
炎症反应过程中活性氧和氮物种（ROS和RNS）的释放会对宿主组织造成损害，即免疫病理学，这是生态免疫学中的一个重要因素。由内源性抗氧化酶（如超氧化物歧化酶SOD和过氧化氢酶CAT）和膳食抗氧化剂（如类胡萝卜素）组成的综合抗氧化系统有助于应对免疫介导的氧化应激。甲壳类动物会储存大量膳食类胡萝卜素，原因尚不清楚。虽然这些色素既是免疫刺激剂又是抗氧化剂，但它们与抗氧化酶之间的相互作用仍不清楚。在此，我们测试了在双足甲壳动物蚤状溞中，膳食补充类胡萝卜素与免疫刺激对免疫防御以及抗氧化酶SOD和CAT活性的相互作用。膳食补充增加了血淋巴中循环类胡萝卜素和血细胞的浓度，而免疫反应则导致循环类胡萝卜素的消耗和血细胞密度的下降。有趣的是，与对照组相比，补充类胡萝卜素的蚤状溞表现出SOD活性下调但CAT活性较高。我们的研究揭示了膳食类胡萝卜素与内源性抗氧化酶之间的特定相互作用，并进一步强调了类胡萝卜素在甲壳动物免疫和/或抗氧化机制进化中的潜在重要性。
