Marin Raul E, Houston Robin, Omanska-Klusek Alicja, Alcaraz Ana, Garcia Jorge P, Uzal Francisco A
private practice, Jujuy, Argentina (Marin)Veterinary Medical Teaching Hospital (Houston), School of Veterinary Medicine, University of California-Davis, Davis, CADepartment of Medicine and Epidemiology (Omanska-Klusek), School of Veterinary Medicine, University of California-Davis, Davis, CACalifornia Animal Health & Food Safety Laboratory, San Bernardino branch (Garcia, Uzal), School of Veterinary Medicine, University of California-Davis, Davis, CACollege of Veterinary Medicine, Western University of Health Sciences, Pomona, CA (Alcaraz).
private practice, Jujuy, Argentina (Marin)Veterinary Medical Teaching Hospital (Houston), School of Veterinary Medicine, University of California-Davis, Davis, CADepartment of Medicine and Epidemiology (Omanska-Klusek), School of Veterinary Medicine, University of California-Davis, Davis, CACalifornia Animal Health & Food Safety Laboratory, San Bernardino branch (Garcia, Uzal), School of Veterinary Medicine, University of California-Davis, Davis, CACollege of Veterinary Medicine, Western University of Health Sciences, Pomona, CA (Alcaraz)
J Vet Diagn Invest. 2015 Jan;27(1):80-5. doi: 10.1177/1040638714559597.
Tunga penetrans is the smallest biting flea known. In cattle, infestation by T. penetrans (tungiasis) typically affects the skin of the distal legs, udder, prepuce, and perianal area. A detailed clinical and pathologic description of bovine tungiasis, together with electron microscopy and molecular diagnostics to establish the identity of the parasite are described. Ninety percent of the cows and heifers and 80% of the bulls in a herd in northwest Argentina had proliferative and ulcerative skin lesions affecting the coronary band, interdigital space, heels, and rudimentary toes of the fore and/or rear limbs, teats, and/or prepuce. These proliferative lesions had multiple large cavities filled with hemorrhagic fluid, necrotic debris, and Tunga spp. parasites. Histologically, the skin showed diffuse papillary epithelial hyperplasia with severe orthokeratotic hyperkeratosis, and it was multifocally ulcerated and inflamed. Multifocally, sections of arthropod parasites were observed embedded in the epidermis and dermis with the posterior end toward the surface. Images of wet mounts and scanning electron microscopy of the parasite showed morphologic characteristics compatible with Tunga spp. Polymerase chain reaction followed by sequencing of the cytochrome c oxidase subunit II and the internal transcribed spacer region indicated 99% homology to published T. penetrans sequences. Tungiasis should be considered as a differential diagnosis for proliferative lesions in skin of cattle.
穿皮潜蚤是已知最小的吸血跳蚤。在牛身上，穿皮潜蚤感染（潜蚤病）通常影响远端腿部、乳房、包皮和肛周区域的皮肤。本文描述了牛潜蚤病的详细临床和病理特征，以及用于确定寄生虫身份的电子显微镜检查和分子诊断方法。阿根廷西北部一个牛群中，90%的母牛和小母牛以及80%的公牛出现了增生性和溃疡性皮肤病变，累及冠状带、趾间间隙、足跟以及前肢和/或后肢的残趾、乳头和/或包皮。这些增生性病变有多个大腔，腔内充满血性液体、坏死碎片和穿皮潜蚤属寄生虫。组织学上，皮肤表现为弥漫性乳头样上皮增生，伴有严重的正角化性角化过度，多处溃疡和炎症。在多处切片中观察到节肢动物寄生虫嵌入表皮和真皮，后端朝向表面。寄生虫的湿片和扫描电子显微镜图像显示出与穿皮潜蚤属相符的形态特征。聚合酶链反应，随后对细胞色素c氧化酶亚基II和内部转录间隔区进行测序，结果表明与已发表的穿皮潜蚤序列有99%的同源性。潜蚤病应被视为牛皮肤增生性病变的鉴别诊断之一。
