Sajadi Maryam, Noroozzadeh Mahsa, Bagheripour Fatemeh, Ramezani Tehrani Fahimeh
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Reproductive Endocrinology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Int J Endocrinol Metab. 2018 Apr 18;16(2):e63135. doi: 10.5812/ijem.63135. eCollection 2018 Apr.
Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in women during the reproductive age. Considering disorders reported in women with PCOS, e.g. infertility, pregnancy complications (premature delivery, caesarean section and spontaneous miscarriages), and hormonal disorders, we hypothesized that uterine contractions in PCOS rats may be different from controls. We aimed to compare uterine contractions in PCOS rats with controls.
Rats in the experimental group were subcutaneously injected with 5 mg of free testosterone on gestational day 20, while controls received solvent. The contractions of isolated uterus in offspring of both groups were recorded by the power lab system, after exposure to carbachol and oxytocin. Two-way analysis of variance was used to compare results between 2 groups.
PCOS rats showed more irregular uterine contractions compared to controls. After exposure to carbachol, frequency, and resting tone in the PCOS rats were significantly increased compared to controls (P = 0.004, P = 0.02, respectively). After exposure to oxytocin, the frequency, resting tone and amplitude of rhythmic contractions did not differ between the 2 groups.
Our study indicates irregular uterine contractions and different mechanical responses of isolated uterus in PCOS rats compared to controls.
多囊卵巢综合征（PCOS）是育龄期女性最常见的内分泌疾病之一。鉴于PCOS女性中报告的疾病情况，如不孕、妊娠并发症（早产、剖宫产和自然流产）以及激素紊乱，我们推测PCOS大鼠的子宫收缩可能与对照组不同。我们旨在比较PCOS大鼠与对照组的子宫收缩情况。
实验组大鼠在妊娠第20天皮下注射5mg游离睾酮，而对照组注射溶剂。两组后代的离体子宫在暴露于卡巴胆碱和缩宫素后，通过功率实验室系统记录其收缩情况。采用双向方差分析比较两组结果。
与对照组相比，PCOS大鼠的子宫收缩更不规律。暴露于卡巴胆碱后，PCOS大鼠的频率和静息张力与对照组相比显著增加（分别为P = 0.004，P = 0.02）。暴露于缩宫素后，两组的频率、静息张力和节律性收缩幅度无差异。
我们的研究表明，与对照组相比，PCOS大鼠的子宫收缩不规律，且离体子宫的机械反应不同。
