van Gent M, Heuvelman C J, van der Heide H G, Hallander H O, Advani A, Guiso N, Wirsing von Kőnig C H, Vestrheim D F, Dalby T, Fry N K, Pierard D, Detemmerman L, Zavadilova J, Fabianova K, Logan C, Habington A, Byrne M, Lutyńska A, Mosiej E, Pelaz C, Gröndahl-Yli-Hannuksela K, Barkoff A M, Mertsola J, Economopoulou A, He Q, Mooi F R
Centre for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM), P.O. Box 1, 3720 BA, Bilthoven, The Netherlands,
Eur J Clin Microbiol Infect Dis. 2015 Apr;34(4):821-30. doi: 10.1007/s10096-014-2297-2. Epub 2014 Dec 20.
Despite more than 50 years of vaccination, pertussis is still an endemic disease, with regular epidemic outbreaks. With the exception of Poland, European countries have replaced whole-cell vaccines (WCVs) by acellular vaccines (ACVs) in the 1990s. Worldwide, antigenic divergence in vaccine antigens has been found between vaccine strains and circulating strains. In this work, 466 Bordetella pertussis isolates collected in the period 1998-2012 from 13 European countries were characterised by multi-locus antigen sequence typing (MAST) of the pertussis toxin promoter (ptxP) and of the genes coding for proteins used in the ACVs: pertussis toxin (Ptx), pertactin (Prn), type 2 fimbriae (Fim2) and type 3 fimbriae (Fim3). Isolates were further characterised by fimbrial serotyping, multi-locus variable-number tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis (PFGE). The results showed a very similar B. pertussis population for 12 countries using ACVs, while Poland, which uses a WCV, was quite distinct, suggesting that ACVs and WCVs select for different B. pertussis populations. This study forms a baseline for future studies on the effect of vaccination programmes on B. pertussis populations.
尽管进行了50多年的疫苗接种，但百日咳仍然是一种地方病，时有流行爆发。除波兰外，欧洲国家在20世纪90年代已用无细胞疫苗（ACV）取代了全细胞疫苗（WCV）。在全球范围内，已发现疫苗株与流行株之间在疫苗抗原方面存在抗原性差异。在这项研究中，对1998年至2012年期间从13个欧洲国家收集的466株百日咳博德特氏菌分离株，通过对百日咳毒素启动子（ptxP）以及ACV中使用的蛋白质编码基因：百日咳毒素（Ptx）、百日咳黏附素（Prn）、2型菌毛（Fim2）和3型菌毛（Fim3）进行多位点抗原序列分型（MAST）来进行特征分析。分离株还通过菌毛血清分型、多位点可变数目串联重复分析（MLVA）和脉冲场凝胶电泳（PFGE）进一步进行特征分析。结果显示，使用ACV的12个国家的百日咳博德特氏菌群体非常相似，而使用WCV的波兰则明显不同，这表明ACV和WCV选择了不同的百日咳博德特氏菌群体。本研究为今后关于疫苗接种计划对百日咳博德特氏菌群体影响的研究奠定了基础。
